Main Article Content
Thirty SSR primer pairs chosen randomly from the sugarcane database SSR primer collection, were used to detect polymorphism in 17 sugarcane accessions. A total of 62 DNA fragments were generated by the 30 primers with an average of about 2.14 bands per primer. Bands yielded by the primer in this study ranged from 1 to 4. The genetic distances were constructed and relationships between accessions were demonstrated graphically using a dendrogram. Genetic similarity ranged from 62.90% to 90.30% and highest genetic similarity of 90.03% was observed among genotypes S-2003-US-118 and S-2003-US-312. From this study it may be concluded that SSRs markers are reliable tool for the investigation of genetic diversity in sugarcane.
Da Maia LC, Palmieri DA, de Souza VQ, Kopp MM, de FIF Carvalho, de Oliveira AC. SSR Locator: Tool for simple sequence repeat discovery integrated with primer design and PCR simulation. International J Pl Genomics. 2008;15:19-28.
Aljanabi SM, Forget L, Dookum A. An improved and rapid protocol for isolation of polysaccharide and polyphenol free sugarcane DNA. Pl Mol Biol Rep. 1999;17: 1-8.
Aitken KS, Jackson PA, McIntyre CL. A combination of AFLP and SSR markers to provides extensive map coverage and identification of homo(eo)logous linkage groups in a sugarcane cultivar. Theor Appl Genet. 2005;110:789-801.
Broun P, Tanksley SD. Characterization and genetic mapping of simple repeat sequences in the tomato genome. Mol Gen Genet. 1996;250: 39-49.
Hokanson SC, McFadden AKS, Lamboy WF, McFerson JR. Microsatellite (SSR) markers reveal genetic identities, genetic diversity and relationships in a Malus 3-domestica borkh. Core subset collection. Theor Appl Genet. 1998;97:671-683.
Eisen JA. Mechanistic basis for microsatellilte instability. In: Goldstein DB, Schlotterer, C (eds) Microsatellites: evolution and applications. Oxford University Press, Oxford. 1999;34–48.
Mclntyre. Verification of the introgression of Erianthus arundinaceus germplasm into sugarcane using molecular markers. Pl. Breed. 2005;124(4):322-328.
Javied M, Ashfaq N, Haider M, Fatima F, Ali Q, Ali A, et al. Agrobacterium-mediated transformation of cotton (Gossypium hirsutum L.) using dmo gene for enhanced tolerance against dicamba pesticide. Biological and Clinical Sciences Research Journal. 2021;2021(1):e009.
Hoisington D, Khairallah M, González D.. Laboratory Protocols: CIMMYT Applied Molecular Genetics Laboratory. CIMMYT, Mexico. 1994;165-173.
Doyle JJ, Doyle JL. Isolation of plant DNA from fresh tissue. Focus. 1990;12:13-15.
Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics. 1978;89(3):583-590.
Chen PH, Pan Y, Chen RR, Xu LP, Chen YQ. SSR marker-based analysis of genetic relatedness among sugarcane cultivars (Saccharum spp. hybrids) from Breeding Programs in China and Other Countries Sugar Tech. 2009;11(4):347-354.
Ahmad M, Ali Q, Hafeez M, Malik A. Improvement for biotic and abiotic stress tolerance in crop plants. Biological and Clinical Sciences Research Journal. 2021; 2021(1):e004.
Aftab A, Haider M, Ali Q, Malik A. Genetic evaluation for morphological traits of coriandrum sativum grown under salt stress. Biological and Clinical Sciences Research Journal. 2021;2021(1):e006.
Bretting PK, Widrlechner MP. Genetic markers and plant genetic resource management. Pl. Breed. Rev. 1995;13:11-86.
Brush SB. Rethinking crop genetic resource conservation. Biol Conserv. 1989;3:1-11.
Burner DM, Pan YB, Webster RD. Genetic diversity of North American and Old World Sacchrum assessed by RAPD analysis. Genet Resour Crop Evol. 1997;44(3):235-240.
Akram A, Arshad K, Hafeez M. Cloning and expression of universal stress protein 2 (usp2) gene in Escherichia coli. Biological and Clinical Sciences Research Journal. 2021;2021(1):e002.
Clegg MT. Molecular diversity in plant populations. In: Brown AHD, Clegg MT, Kahler AL, Weir BS (eds) Plant population 682 genetics, breeding, and genetic resources. Sinauer Associates Inc., Sunderland, Massachusetts. 1990;98-115.
Collard BCY, Jahufer MZZ, Brouwer JB, Pang ECK. An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: The basic concepts. Euphytica. 2005;142:169-196.
Cordeiro GM, Pan YB, Henry RJ. Sugarcane microsastellites for the assessment of genetic diversity in sugarcane germplasm. Pl Sci. 2003;165(1):181-189.
Ali M, Rafique F, Ali Q, Malik A. Genetic modification for salt and drought tolerance in plants through SODERF3. Biological and Clinical Sciences Research Journal. 2020; 2020(1):e022.