MICROSATELLITE (SSR) MARKERS A TOOL; FOR GENETIC DIVERSITY ASSESSMENT AMONG SUGARCANE ACCESSIONS

Main Article Content

SOHAIL AKHTER
SALSABEEL RAUF
BELQEES AKHTER
MARIA GHIAS
NAJMA PARVEEN
IQRA IBRAR
SAJJAD ALI GILL
QURBAN ALI

Abstract

Thirty SSR primer pairs chosen randomly from the sugarcane database SSR primer collection, were used to detect polymorphism in 17 sugarcane accessions. A total of 62 DNA fragments were generated by the 30 primers with an average of about 2.14 bands per primer. Bands yielded by the primer in this study ranged from 1 to 4. The genetic distances were constructed and relationships between accessions were demonstrated graphically using a dendrogram. Genetic similarity ranged from 62.90% to 90.30% and highest genetic similarity of 90.03% was observed among genotypes S-2003-US-118 and S-2003-US-312. From this study it may be concluded that SSRs markers are reliable tool for the investigation of genetic diversity in sugarcane.

Keywords:
SSR, polymorphism, genetic diversity

Article Details

How to Cite
AKHTER, S., RAUF, S., AKHTER, B., GHIAS, M., PARVEEN, N., IBRAR, I., GILL, S. A., & ALI, Q. (2021). MICROSATELLITE (SSR) MARKERS A TOOL; FOR GENETIC DIVERSITY ASSESSMENT AMONG SUGARCANE ACCESSIONS. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 22(59-60), 1-7. Retrieved from https://www.ikprress.org/index.php/PCBMB/article/view/7087
Section
Original Research Article

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