PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Abstract

Thirty SSR primer pairs chosen randomly from the sugarcane database SSR primer collection, were used to detect polymorphism in 17 sugarcane accessions. A total of 62 DNA fragments were generated by the 30 primers with an average of about 2.14 bands per primer. Bands yielded by the primer in this study ranged from 1 to 4. The genetic distances were constructed and relationships between accessions were demonstrated graphically using a dendrogram. Genetic similarity ranged from 62.90% to 90.30% and highest genetic similarity of 90.03% was observed among genotypes S-2003-US-118 and S-2003-US-312. From this study it may be concluded that SSRs markers are reliable tool for the investigation of genetic diversity in sugarcane.

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