In vitro SHOOT MULTIPLICATION AFFECTED BY LIGHT AND CULTURE SYSYEM IN POTATO
MOHAMMED J. H. Al-SHAREEFI *
Agriculture College, University of Al-Qasim Green, Iraq.
MUSLIM ABD ALI ABDULHUSSEIN
Agriculture College, University of Kufa, Iraq.
JAMAL AHMED ABBASS
Agriculture College, University of Kufa, Iraq.
*Author to whom correspondence should be addressed.
Abstract
This study aimed to investigate the effect of the light sources and culture system on in vitro growth and multiplying shoots of potato. The plants were exposed to different spectral light sources for 16 hd-1 photoperiod under the following three treatments: white light (Florescent) the control, red (R16) and blue (B2) LEDs and red (R18) and blue (B2) LEDs. Also, this study comprised three treatments of culture systems: temporary immersion system (TIS) which contains 500 ml of MS media in each container, we used 40 segments of two nodes with an immersion and frequency time of two minutes each two hours. Solid and liquid culture systems which contains 50 mL of MS media in each vessel, we used 4 segments of two nodes. The results showed the excelled of the plants growing under (LED R16: B2 + TIS) showed the highest rate of shoots number, fresh weight and dry weight percentage. While, the treatment (LED R18: B2 + TIS) which achieved the highest rate of shoots length. The treatment (LED R18: B2 + solid culture) achieved the highest rate of chlorophyll content.
Keywords: Potato, LEDs, temporary immersion system, TIS