Short Research Articles

VARIATION IN ANTIBACTERIAL AND ANTIOXIDANT POTENCY OF TEA AND COFFEE EXTRACTS

SNIGDHA SINGH, NEHA MADDHESHIYA, SHAILENDRA KUMAR TRIPATHI, MADHULIKA SINGH

Asian Journal of Microbiology and Biotechnology, Volume 3, Issue 1, Page 1-7

Antimicrobial property of tea and coffee is well known. Green tea is known for its therapeutic properties. In this study antibacterial activity of green tea, black tea and coffee extracts were evaluated against isolated bacterial species from healthy human saliva, which after identification was found to be Streptococcus sp. Antioxidant and antimicrobial property of green, black tea and coffee can be exploit as an alternative of therapeutic drugs, as many of the microbial species had discovered the escape mechanism against them.

Original Research Article

SEROPREVALENCE OF SYPHILIS AMONG PREGNANT WOMEN RECEIVING ANTENATAL CARE AT MEDICAL CENTRE, MARARABA, NASARAWA STATE

L. Y. ADOGO, C. E. ANADUAKA, A. B. AJIDE

Asian Journal of Microbiology and Biotechnology, Volume 3, Issue 1, Page 8-14

This study was carried out to determine the seroprevalence of syphilis among pregnant women receiving antenatal care (ANC) at the Medical Centre, Mararaba, Nasarawa State. Three hundred (300) pregnant women were recruited for this cross-sectional descriptive study carried out from June to July, 2017. 2ml of blood sample was collected via venipuncture, transferred into EDTA bottles and centrifuged at 1,500rpm for 5 minutes. The screening for syphilis was carried out using qualitative Rapid test kits. The prevalence rate of 4.3% at 0.05 confidence limit was obtained from this study. The prevalence of 2% was recorded among pregnant women within the age range of 22-29. There was no significant relationship between syphilis infection and age (P = 0.432).The infection was more prevalent among pregnant women in their second trimester with the prevalence rate of 2.3%. There was no significant relationship between syphilis infection and trimester (P = 0.432). 1% prevalence was recorded among pregnant women who had a history of blood transfusion. There was no significant relationship between syphilis infection and history of blood transfusion (P = 0.432). The prevalence rate recorded in this study was high when compared to the national prevalence of 0.7% reported in 2014.  Maternal syphilis puts the foetus at risk of congenital syphilis, therefore routine screening for syphilis in antenatal clinics should be encouraged to prevent mother to child transmission of syphilis.

Original Research Article

ANTIMICROBIAL ACTIVITY OF PLANT BARK USED AS NATURAL CHEWING STICK AGAINST MOUTH FLORA AND LABORATORY BACTERIA

RUCHI J. PATEL, JIGAR V. SUTHAR, RAJESHKUMAR PATEL, KANAGEE J. GAJJAR, JPAN G. BRAHMBHATT

Asian Journal of Microbiology and Biotechnology, Volume 3, Issue 1, Page 15-23

Antimicrobial activity denotes the inhibition activity or killing activity of chemical or extracts component against living organisms. Plants have the variety of these kinds of components which can be utilized to inhibit the pathogenic activity of some pathogens in our routine life. Here we took three plants (Neem, Babool and Miswak) bark which are used as chewing sticks, to make three different (Acetone, Methanol and Aqueous) extracts. By well diffusion method, these all extracts were checked against extracted mouth flora and common laboratory bacteria (E. coli, S. typhi, B. subtilis, P. vulgeris and Micrococcus). Phytochemical study of these bark juices were also done. Our data shows that the acetone extract of Babool is most effective among all other extract we tested.

Original Research Article

PHOSPHATE SOLUBILIZING ACTIVITY OF Rhizobium sp. ISOLATED FROM Vigna mungo GROWN IN SEMI-ARID REGION

RICHA JINDAL, DIPJYOTI CHAKRABORTY

Asian Journal of Microbiology and Biotechnology, Volume 3, Issue 1, Page 34-39

Rhizobium sp. was isolated from Vigna mungo RBU 38 grown in a semi-arid region of Rajasthan, India. The isolated Rhizobium sp. produced the phytohormone, IAA, ammonia and has the ability for phosphate solubilization. It can be used as biofertilizer for future application in agriculture for increased crop yield.

Review Article

Mycobacterium tuberculosis: GENE AND GENOME ANALYSIS

SHREE RAM LAMICHHANE, NATALIE MILIC

Asian Journal of Microbiology and Biotechnology, Volume 3, Issue 1, Page 24-33

The prevalence of tuberculosis (TB) continues to be high, and it is a major threat in global public health. Mycobacterium family has over 60 species, however, only Mycobacterium tuberculosis, Mycobacterium leprae, Mycobacterium Africanum and Mycobacterium avium are pathogenic to humans. Although other animals can also get infected, humans are the only known host for Mycobacterium tuberculosis. Once the immune system is compromised, the microorganism can cause infection or can be reactivated if previously infected with the bacteria. The availability of complete genome sequencing of both laboratory and virulent strain of M. tuberculosis has provided researchers a wealth of new knowledge and greater understanding about this microorganism. This article summarizes the nature of these infectious bacteria at a molecular level, taking into account the recent advancements in sequencing and drug resistance.