Optimization of Plant Growth Regulators for Efficient in vitro Morphogenesis and Plantlet Regeneration in Bambusa vulgaris
Rahul More
Department of Genetics & Plant Breeding, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, Madhya Pradesh, 474002, India.
Deepa Bhatt
Department of Plant Molecular Biology & Biotechnology, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, Madhya Pradesh, 474002, India.
M.K. Tripathi *
Department of Genetics & Plant Breeding, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, Madhya Pradesh, 474002, India, Department of Plant Molecular Biology & Biotechnology, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, Madhya Pradesh, 474002, India and Zonal Agricultural Research Station, Morena, 476001, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, M.P., India.
Ankit Pandey
KNK-College of Horticulture, Mandsaur 458001, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, M.P., India.
Riya Mishra
Department of Genetics & Plant Breeding, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, Madhya Pradesh, 474002, India.
D. K. Payasi
RAK College of Agriculture, Sehore, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, M.P., India.
Prerna Parihar
Department of Plant Molecular Biology & Biotechnology, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, Madhya Pradesh, 474002, India.
Shashank Bhargav
Department of Plant Molecular Biology & Biotechnology, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, Madhya Pradesh, 474002, India.
Purnima Singh
Department of Plant Molecular Biology & Biotechnology, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, Madhya Pradesh, 474002, India.
Mohini Sharma
Department of Plant Molecular Biology & Biotechnology, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, Madhya Pradesh, 474002, India.
Nishi Mishra
Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.
Shrankhla Mishra
Department of Agricultural Engineering, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, Madhya Pradesh, India, 474002, India.
Ramawatar Choudhary
Department of Plant Molecular Biology & Biotechnology, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, Madhya Pradesh, 474002, India.
Sanjeev Sharma
Department of Genetics & Plant Breeding, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, Madhya Pradesh, 474002, India.
Sharad Tiwari
Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.
*Author to whom correspondence should be addressed.
Abstract
Bamboo is the world’s sturdiest and multipurpose woody plant proficient in providing environmental, commercial, and livelihood safety to society, and is also recognized as ‘Green Gold’, ‘Miracle plant’. It has conventionally been propagated through seed or vegetative methods, but these methods are beset with several problems. Functioning arrangements prerequisite to be implemented to protect bamboo from excessive pruning and to inspire its sustainable growth and development to meet demands and ecological safeguarding. Thus, during the current investigation, a protocol has been established to regenerate plantlets through direct in vitro organogenesis from inoculated nodal segments excised from thee-year-old plants. Nutrient medium MS3B.5Kn.5G encompassing MS basal medium supplemented with 3.0 mgl-1 BAP in combination with 0.5 mgl-1 Kn and 0.5 mgl-1 GA3 proliferated maximum shootlets (78.03%) with higher number (7.0) and a bigger length (6.32 cm). The highest rooting efficiency (77.78%) with higher root number (4.07) and length (9.50cm) was evident on MS medium amended with 5.0 mgl-1 IBA. Whereas, maximum survival percentage (86.67) of regenerants was recorded on potting mixture comprising sand, soil, and farmyard in a 1:1:1 ratio under greenhouse conditions after six weeks of primary hardening. The in vitro raised regenerants were accustomed and established effectively under the field conditions. Although the characters were not scored quantitatively, regenerated plantlets appeared phenotypically normal and similar to the mother plants.
Keywords: Bambusa balcooa, in vitro propagation, morphogenesis, nodal segment, plantlet regeneration