Molecular Characterization of New Plant Types (NPTs) of Rice Developed at JNKVV, Jabalpur (M.P), India Using SSR Markers
Yogendra Singh *
Department of Genetics & Plant Breeding, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004 (M.P), India.
G. K. Koutu
Department of Genetics & Plant Breeding, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004 (M.P), India.
M. J. Kujur
Department of Genetics & Plant Breeding, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004 (M.P), India.
Ekta Hedau
Department of Genetics & Plant Breeding, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004 (M.P), India.
*Author to whom correspondence should be addressed.
Abstract
Rice (Oryza sativaL.)is one of the three major food crops of the world and considered to be the staple diet of more than half of the population worldwide. It is the most widely consumed staple food across Asia and it accounts. Improving rice yield potential to meet the food demand of the growing population has been the main breeding objective in many countries. To achieve this, the New Plant Type (NPT) approach has been used in breeding programs, which includes reduced tillering, large panicles, high grain density, high fertile spikelets per panicle, longer grain filling period, increased harvest index etc.Simple sequence repeat (SSR) markers are highly effective co-dominant markers that target specific loci within the genome and are commonly employed in molecular studies. These markers are widely utilized in various applications, including genotypic analysis, molecular mapping, cultivar identification, hybridity assessment, and the evaluation of gene pool diversity. SSR markers are particularly valuable in the identification of traits with economic significance due to their high reproducibility and species-specific nature. In this study, 15 SSR markers were utilized to perform PCR-based molecular analysis of 128 New Plant Types (NPTs) of rice developed by JNKVV, Jabalpur (M.P.)The polymorphism of markers was scored as 0 and 1 depicting absence and presence of band, the binary data was processed and rooted Phylogeny tree (dendrogram) were prepared using “DARwin 6.0” software. The Phylogenic Tree based on SSR Markers clearly showed that 128 NPT lines were broadly distributed in three major groups A, B and C having 7, 49 and 72 NPT lines respectively. The group A is further divided in to A1 and A2 sub groups comprising of 3 and 4 NPT lines respectively . The group B is further divided in to B1 and B2 sub groups comprising of 10 and 39 NPT lines respectively (Fig. 1). Group C was further subdivided into C1 and C2 having 23 and 49 NPT lines respectively.
Keywords: Rice, new plant type (NPT), SSR marker, molecular characterization