Anti-cancer Effects of Plant-based herbal Formulation on Vero and Human Colorectal Cancer Cell Lines
S. Selvakumar *
Department of Industrial BioTechnology, Bharath Institute of Higher Education and Research, Chennai-600073, India.
Shifa Raihaan Siddique
Department of Industrial BioTechnology, Bharath Institute of Higher Education and Research, Chennai-600073, India.
Shiny Fathima H.
Department of Industrial BioTechnology, Bharath Institute of Higher Education and Research, Chennai-600073, India.
*Author to whom correspondence should be addressed.
Abstract
The present study aims to search for excellent antineoplastic agents avertable to human colorectal malignancies. In vitro assay for cytotoxicity activity (3‑[4,5‑dimethylthiazol‑2‑yl] ‑2,5‑diphenyltetrazolium bromide assay) (MTT Assay), DNA fragmentation was analysed by single gel electrophoresis and the activities of certain tumor specific marker enzymes such as acid phosphatase, aspartate aminotransferase, lactate dehydrogenase, alanine aminotransferase, alkaline phosphatase is also analyzed. Our results envisage that the non-cytotoxic nature of polyherbal formulation on Vero and human colorectal carcinoma [HT – 29] cell lines and the poly herbal formulation possess the strong anticancer activities on human colorectal carcinoma cell lines. The evaluation and the discovery of new anticancer agents is a long-term process that encompasses many steps. The step broaches with the screening for anticancer properties, followed by the isolation and identification of bioactive compounds obliged to anticancer properties, toxicity estimation of the isolated compounds, and finally in vivo anticancer activity testing to verify the aptitude of the compounds.
Keywords: Anticancer agent, non-coding RNA, colorectal tumours, heterozygosity, microsatellite instability, polyherbal formulation