Efficient Shoot Induction from Apical Meristem Culture in Olive (Olea europaea L.)
Firoozeh Chamandoosti *
Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.
*Author to whom correspondence should be addressed.
Abstract
Olive propagation by tissue culture method is an efficient method for rapid asexual propagation, production of disease-free plants, and access to propagating materials throughout the year for olive plants. In this study, meristematic tissue explants of the Koroneiki cultivar from olive trees were cultured in different basal media included MS (Murashige and Skoog), OM (Olive Medium) and DKW (Driver and Kuniyki), without plant growth regulators. Then, the explants were cultured in basal media supplemented with auxins [NAA (Naphthalene acetic acid, IAA (Indole acetic acid) and IBA (Indole butyric acid)] and cytokinins [BA (Benzyl adenine), KIN (Kinetin), 2ip (Isopentenyl adenine), TDZ (Thidiazuron), and ZEA (Zeatin)]. The superiority of DKW basal media and ZEA growth regulator for inducing branching and longitudinal growth of branches for the Koroneiki cultivar was proven. With increasing concentrations of growth regulators, especially BA, was the dominant response than other responses such as meristem growth and shoot induction.
Keywords: Apical meristem, driver and kuniyki medium, olive, zeatin