In-vitro Regeneration Potential of Tinospora cordifolia (Wild.) Miers Using Nodal Explant Grown in Murashige and Skoog (MS) Medium
Soni Kushwaha
Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, India.
Padmanabh Dwivedi
Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, India.
Akash Kanaujia
Department of Horticulture, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, India.
Pooja Belwal
Department of Horticulture, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, India.
Nitin Yadav
*
Department of Horticulture, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, India.
Amit Kushwaha
Department of Soil Science and Agricultural Chemistry, Institute of Agricultural Sciences, BHU, Varanasi-221005, India.
Basant Kumar Dadrwal
Department of Plant Physiology, Sri Karan Narendra Agriculture University, Jobner, Rajasthan-303329, India.
*Author to whom correspondence should be addressed.
Abstract
This study aims to analyze the effects of various shoot-promoting growth hormones on the micropropagation of above-ground plant parts of Tinospora cordifolia. Tinospora cordifolia is a valuable medicinal plant with diverse therapeutic properties, including immunomodulatory, anti-inflammatory, and antioxidant activities. The plant's extracts are in high demand for pharmaceutical applications, and micropropagation can ensure a consistent supply of high-quality plant material. The experiment will be conducted applying Murashige and Skoog (MS) media which is augmented with varying concentrations of: (BAP), Thidiazuron (TDZ) and Kinetin. Various parameters are recorded throughout the experiment, including the number of days to primordia initiation, shoot count, node count, shoot length, leaf count, and intensity of callus formation. Among the three hormones tested, BAP and kinetin exhibited a more favorable response compared to the other hormone in MS media.
Keywords: MS (Murashige and Skoog) medium, Tinospora cordifolia, 6-Benzylaminopurine, TDZ, In-vitro, kinetin