In vitro MULTIPLE SHOOT INDUCTION OF Citrus aurantifolia
PUNYAKISHORE MAIBAM *
College of Food Technology, Central Agriculture University, Manipur-795004, India.
VAIDURYA PRATAP SAHI
Department of Plant Breeding and Genetics, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj-211007, India.
*Author to whom correspondence should be addressed.
Abstract
The present study provides an efficient and simple method for the micropropagation of C. aurantifolia which can be effectively used for its mass propagation. Of all the different concentrations used, the maximum number of shoots was initiated in the medium supplemented with 1.5 mg/l of BAP. The medium supplemented with 1.5mg/l BAP and 1.5 mg/l KN was proven to be the best for shoot induction with maximum response of explants (79.57 %) as well as the maximum number of shoots per explant with shoot length. However, there was no significant response from the explant inoculated in the medium supplemented with cytokinin (KN and BAP) in combination with auxin (2, 4-D and NAA). NAA was found to be more effective for root induction than IAA. The regenerated shoots cultured in MS medium supplemented with 1.5 mg/l NAA resulted in maximum response of 83.52%.
Keywords: Citrus aurantifolia, micropropagation, mutiple shoot induction, 6-benzyl aminopurine (BAP), kinetin (KN), indole 3-acetic acid (IAA), α-naphthaleneacetic acid (NAA)