CHEMICAL COMPOSITION BY GC-MS, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF PEELS AND LEAVES EXTRACTS FROM Citrus limon, Citrus sinensis AND Citrus reticulata
HADJER BOUSSOUSSA *
Laboratoire des Sciences Fondamentales, Equipe Produits Naturels et Activités Biologiques, Université Amar Telidji, Laghouat, BP 37G Laghouat, Algeria.
IHCEN KHACHEBA
Department of Biology, Université Amar Telidji, Laghouat, BP 37G, Laghouat, Algeria and Department of Chemistry and QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal .
SARA BOUKHALKHAL
Department of Biology, Université Amar Telidji, Laghouat, BP 37G, Laghouat, Algeria and Department of Chemistry and QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal.
YASMINE LAMRI
Department of Biology, Université Amar Telidji, Laghouat, BP 37G, Laghouat, Algeria and Department of Chemistry and QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal.
AHLAM BOUTI
Department of Biology, Université Amar Telidji, Laghouat, BP 37G, Laghouat, Algeria and Department of Chemistry and QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal.
ARTUR M. S. SILVA
Department of Biology, Université Amar Telidji, Laghouat, BP 37G, Laghouat, Algeria and Department of Chemistry and QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal.
MOHAMED YOUSFI
Department of Biology, Université Amar Telidji, Laghouat, BP 37G, Laghouat, Algeria and Department of Chemistry and QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal.
*Author to whom correspondence should be addressed.
Abstract
Citrus family especially lemon, mandarin and orange, have reached important attention due to the presence of several components with different therapeutic properties. The leaves and peels of non-polar extracts from bio-citrus cultivars were investigated for phytochemical analysis, antibacterial and antioxidant capacity. The DPPH (1,1-Diphenyl-2-picrylhydrazyl) activity and phosphommolybdenum antioxidant effect values from leaves and peels ranged from 0 to 12.79% and 0.006 to 0.14 mg/ml AAeq respectively. Results showed significant differences in antioxidant properties of leaves and peels extracts. The leaves of mandarin exhibited a higher antioxidant activity than the other extracts from lemon and orange. Among different components identified from the organic extracts, limonene, myrcene, and benzopyran were major components in all samples.
Keywords: Citrus limon, Citrus sinensis, Citrus reticulata, GC-MS analysis, antioxidant activity, antibacterial activity