PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Halloki village Lahore was explored for freshwater algae and 7 samples were collected from different sites (Freshwater ponds, marshy areas, irrigated channels, watered field, road side streams, etc) during October 2020 to April 2021. Total nineteen genera and thirty four species of algae belonging to three kingdoms; Monera, Protista and Protoctista were recorded for the very first time from Halloki village Lahore, Pakistan, rest of them have previously been reported other sites of Lahore (lil. cit). Kingdom Monera included one phylum Cyanophycota (8.82%) containing 1 class, 1 order, 2 families, 2 genera and 3 species i.e. Oscillatoria irrigua, O. tenuis and Anabaena aequalius. Kingdom Protista was found to be most abundant having three phyla; Volvophycota (29.40%), Euglenophycota (2.94%) and Bacillariophycota (29.41%) containing 4 classes, 6 orders, 11 families, 13 genera and 21 species i.e. Crucigenia quadrata, Closteridium lunula, Palmodictyon viride, P. varium, Cosmarium subcreantum, Closterium lanceolatum, C. acerosum, C. parvulum, C. cornu var. arcum, C. lunula var. lunula f. biconvexum, Euglena polymorpha, Cymbella affinis, Nitzchia vasnii, N. palea, Acanthes trigibba, Navicula disjuncta, N. microspora, N. exigua, Anomoeoneis exilis, Synedra ulna and Melosira varaians. Kingdom Protoctista included one phylum Chlorophycota (29.40%) containing 2 classes Ulvophyceae (11.76%) and Zynemophyceae (17.64%) respectively, 4 orders, 4 families, 4 genera and 10 species i.e. Microspora ameona, M. crassior, M. pachyderma, Ulothrix subconstricta, Oedogonium nodulosum, Spirogyra condensata, S. aplanospora, S. intorta, S. bullata and S. acquinoctialis.