POLYPHENOL CONTENT AND ANTIOXIDANT ACTIVITIES OF THE SHOOTS EXTRACTS FROM Rubus idaeus L
DJAMILA AMENI *
Department of Common Core, Institute of Sciences, University Centre Abdellah Morsli Tipaza, Algeria.
SALIHA DJIDEL
Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biochemistry, Faculty of Nature and Life Sciences, University Ferhat Abbes Setif, Algeria.
MERIEM DJARMOUNI
Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life Sciences, University Ferhat Abbes Setif, Algeria.
SEDDIK KHENNOUF
Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biochemistry, Faculty of Nature and Life Sciences, University Ferhat Abbes Setif, Algeria.
LEKHMICI ARRAR
Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life Sciences, University Ferhat Abbes Setif, Algeria.
ABDERRAHMANE BAGHIANI
Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life Sciences, University Ferhat Abbes Setif, Algeria
*Author to whom correspondence should be addressed.
Abstract
Biomolecules are largely found at different parts in divers medicinal plants. This study aims to quantify polyphenols and some biological properties of the areal part of Rubus idaeus L. plant. The shoots of Rubus idaeus L. were extracted by a methanol-water mixture. The antioxidant capacity was estimated by different in vitro assays ; linoleic acid emulsion model system, ß-carotene-linoleate model system, DPPH reduction, free radical-scavenging activity, and reducing ability assay. The highest levels of polyphenolic compounds was observed in the ethyl acetate extract (EAE). In linoleic acid test, chloroform extract (CHE) had the strongest antioxidant in comparison with butylated hydroxyl toluene (BHT) (64. 549 ± 0. 007%). In b-carotene/linoleic acid bleaching assay, the crude extract (CE) had the highest antioxidant capacity. The results showed that EAE showed the strongest antioxidant activity as measured by radicals scavenging activities and reducing power.The present work provide evidence that the areal part extracts of R. idaeus has remarkable antioxidant activities.
Keywords: Rubus idaeus L., polyphenols, free radicals, antioxidants, lipid oxidation