COMPARATIVE INHERITANCE INDEX IN ELITE WHEAT LINES THROUGH RAPD MARKERS
ARUBA LAIQUE
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.
TEHREEM MARIAM
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.
AYMUN IFTIKHAR
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.
RUBAB RAZZAQ
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.
TANZEELA ZAFAR
Center for Agriculture Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan.
NAZAR HUSSAIN KHAN
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.
I. E. AMIR BIBI
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.
SEHAR NAWAZ
Center for Agriculture Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan.
FOZIA SALEEM
Center for Agriculture Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan.
SIDRA NIAZ
University of Agriculture, Faisalabad, Pakistan.
M. AHSAN IQBAL *
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
The experiment was conducted at Centre for Agricultural Biochemistry and Biotechnology (CABB), university of Agriculture Faisalabad. The 15 genotypes were evaluated for heredity. Through 15 primers, 1181 bands were amplified using PCR based identifiers-rapid DNA markers. FSD-2008, KOHISTAN97, 4072, 9021, 9189, 9244, 9272, 9277, 9381, 9428, 9249, 9451, 9452, 9481 and 9476 are the genotypes which were evaluated to check heredity similarities. The elevated number of bands were produced by K7- (88) and lowest by primer 17 that were 16. FSD-2008 and 9481 showed 82% similarities while 9381 and 9249 depicted 81% heredity. Similarly, KOHISTAN-97 and 9277 showed 81% heredity. 88% heredity was found among 4072 and 9021 accessions.
Keywords: PCR (polymerase chain reaction), TBE (tris borate acid EDTA), EDTA (ethylene diamine tetra acetic acid), RAPD (randomized polymorphic DNA markers), CTAB (cetyltrimethylammonium bromide)