PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

In this study, we isolated and purified compounds from the n-Butanol segment of seed Concave white Lotus variety (N. nucifera) collected at Tinh Tam Lake, Thua Thien Hue province based on classical column chromatographic methods. Research results have obtained three compounds namely: Nuciferin (NH2 : C₁₉H₂₁NO₂), Armepavine (NH8 : C₁₉H₂₃O₃N), and Anonaine (NH11 : C₁₇H₁₅NO₂). At the concentration of 100 µg/mL, Nuciferin was the highest DPPH free radical scavenging activity substance reaching of 94.620 ± 0.657%, followed by Armepavine (74.019 ± 2.400%) and Anonaine (61.151 ± 2.349 %). The IC₅₀ values obtained from these substances were 30.031 ± 1.506 µg/mL (Nuciferin), 43.093 ± 14.215 µg/mL (Armepavine) and 68.217 ± 6.214 µg/mL (Anonaine) respectively. The cytotoxicity results of three active ingredients showed the very low activity of the investigated substances at the studied concentrations. The IC₅₀ value cannot be determined in the concentration range from 0.8; 4; 20 and 100 µg/mL. At a concentration of 100 µg/mL, Nuciferin gave the best inhibitory activity against the human gastric cancer cell line MKN7 (23.800%), Armepavine showed the highest inhibitory activity against the oral carcinoma cell line KB (12.320%) and Anonaine showed the best inhibitory activity against the human skin cancer cell line SK-Mel-2 (19.720%). Our results initially show the prospect of using Concave white Lotus seeds as functional foods to support and improve human health.