ANTIDIABETIC AND ANTIOXIDANT ACTIVITIES OF POLYHERBAL FORMULATION IN ALLOXAN INDUCED DIABETIC WISTAR RAT
DEROUICHE SAMIR *
Department of Cellular and Molecular Biology, Faculty of the Sciences of Nature and Life, El Oued University, El-Oued, Algeria and Laboratory of Biodiversity and Application of Biotechnology in the Agricultural Field, El Oued University, El-Oued, Algeria
BENMOUSSA OUISSAM
Department of Cellular and Molecular Biology, Faculty of the Sciences of Nature and Life, El Oued University, El-Oued, Algeria
*Author to whom correspondence should be addressed.
Abstract
Objective: To investigate the effect of polyherb (formulated by some plants) on physiological, biochemical alterations and oxidative stress induced by experimental diabetes in rats.
Materials and methods: Twenty female albino Wistar rats were randomly divided into 4 groups (n=5); Control, Polyherb, diabetic rats and Diabetic rats treated with polyherb. All types of treatments were given to rats orally for 21 days.
Results: In vitro phytochemical analysis results revealed that polyherb contains phenols, flavonoids, alkaloids, terpenoids, tannins and steroids. In vivo results, in diabetic group showed an alteration in lipid profile and biochemical parameters. While, results showed an increase in MDA level and a decrease in GSH and SOD levels compared to control. Moreover, the hematological parameters revealed that Diabetes induce a significant diminution (P<0.01) of RBC, MCV and HCT levels. In the other hand, histopathological analysis noted an alteration in pancreas tissues of diabetic rats group compared to control. However, treatment of Diabetic rats by Polyherb ensured a partial amelioration and correction of the previous parameters.
Conclusion: Use of Polyherb appears to be the dominant limited of development and complications of diabetes which can have a benefic effect against others diseases which oxidative stress plays an important role.
Keywords: Diabetes, oxidative stress, polyherb, rats