THE ANTIBACTERIAL EFFECT OF Uva ursi PLANT TINCTURE AGAINST SOME UROPATHOGENS
LAILA SUMREEN *
Department of Homeopathic Medical Sciences, University College of Conventional Medicine, Faculty of Medicine and Allied Health Sciences. The Islamia University of Bahawalpur, Pakistan.
GHAZALA SHAHEEN *
Department of Eastern Medicine. University College of Conventional Medicine. Faculty of Medicine and Allied Health Sciences, The Islamia University of Bahawalpur, Pakistan.
TAHIRA SHAMIM *
Department of Eastern Medicine. University College of Conventional Medicine. Faculty of Medicine and Allied Health Sciences, The Islamia University of Bahawalpur, Pakistan.
TAYYEBA REHMAN *
Faculty of Medicine and Allied Health Sciences, University College of Conventional Medicine, The Islamia University of Bahawalpur, Pakistan.
RIDA TANVEER *
Department of Homeopathic Medical Sciences, University College of Conventional Medicine, Faculty of Medicine and Allied Health Sciences. The Islamia University of Bahawalpur, Pakistan.
JAFIR HUSSAIN SHIRAZI *
Faculty of Pharmacy, Department of Pharmaceutics, The Islamia University of Bahawalpur, Pakistan.
QAZI ADNAN *
Faculty of Pharmacy, Department of Pharmaceutics, The Islamia University of Bahawalpur, Pakistan.
FAHEEM HADI *
Institute of Molecular Biology and Biotechnology, University of Lahore, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
Urinary tract infections are most significant cause of disease in females and children. Homoeopathic mother tinctures are clinically used for the management of different pathological conditions however; there are several controversial theories for their effectiveness. The objective of this study was to assess the antibacterial effect of Uva ursi tincture in comparison with antibiotic ampicillin. In this study, well diffusion method was used to determine the antibacterial activity of homoeopathic plant tincture Uva ursi against four uropathogenic bacterias (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae). Standard drug as positive control Ampicillin (Novadit injectable) used in concentration of 200mg/100ml and alcohol 70% was taken as negative control group. Inhibition zones were observed after 24-hour incubation. Standard drug Ampicillin showed significant antibacterial activity for Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae with zone of inhibition 40, 32, 30 and 22 mm respectively. Uva ursi mother tincture has maximum antibacterial activity against Staphylococcus aureus with zone of inhibition range up to 31 mm and least against Klebsiella pneumoniae zone of inhibition range up to 13mm, showed less susceptibility as compared to Staphylococcus aureus. Uva ursi 30C (potency) showed maximum inhibition against Staphylococcus aureus was 13mm and least against Klebsiella pneumoniae 4mm. The results showed that Uva ursi tincture have significant result against some common uropathogenic bacteria.
Keywords: Mother tincture, antibacterial, Uva ursi, uropathogens