In-vitro ANTIOXIDANT ACTIVITY OF Dianthus caryophyllus L. ETHANOLIC EXTRACT
MOUNIRA MERGHEM *
Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Natur and Life Sciences, Ferhat Abbas University, Sétif-1, Algeria
NAWEL MEROUANI
Laboratory of Applied Microbiology, Faculty of Natur and Life Sciences, Ferhat Abbas University, Sétif-1, Algeria
WAFA NOUIOUA
Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Natur and Life Sciences, Ferhat Abbas University, Sétif-1, Algeria.
*Author to whom correspondence should be addressed.
Abstract
Carnation (Dianthus caryophyllus L) which considered as one of the most important flower, gardens aromatic crop plants originates in the Mediterranean region. This study was designed to evaluate the phenolic, flavonoid contents and antioxidant activity of Dianthus caryophyllus ethanolic extract. However, DPPH and β-carotene bleaching methods were applied to evaluate the in vitro antioxidant activity. The polyphenolic and flavonoids contents of Dianthus caryophyllus ethanolic extract were found to be 188.217 μg GAE/mg extract and 22.2 μg QE/mg extract, respectively. The scavenger effect of the extract against DPPH radicals showed IC50 value of 0.11 ± 0.001 mg/ml. The β-carotene bleaching assay indicated a strong inhibition percentage of the lipid peroxidation with a value of 81.075±1.436. It can be concluded that D. caryophyllus extracts possessed higher antioxidant activities.
Keywords: Dianthus caryophyllus, antioxidant activity, polyphenols, DPPH scavenging, β-carotene