PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

In this study the sorption properties of a new multifunctional hemosorbent (G) based on natural silk fibroin were evaluated.The properties of the hemosorbent was studied on experimental models in three media: adsorption of water vapor, benzene vapor, and vitamin B₁₂. From the isotherms of water vapor adsorption by silk fiber and hemosorbent, it was found that after hydrolysis of silk fiber, its water vapor absorption properties were reduced by two times. In the hydrolysis of alkaline fibers, fibroin is cleared of sericin and related substances, and its hydrophilicity decreasesd up to 2 times. From isotherms of benzene vapor adsorption by silk fiber and hemosorbent, silk fiber practically did not adsorb benzene, and hemosorbent adsorbed 64 times more benzene molecules. It was observed that the adsorption of vitamin B12 showed that the amount of unsorbed vitamin decreased with increased adsorption time. Moreover, with an increase in time, the adsorption process slows down significantly. The optimal time for immobilization of a fibrous polyfunctional hemosorbent based on hydrolyzed fibroin, placed in a column with vitamin B₁₂, was 10 minutes (95%). In conclusion, this study showed that after hydrolysis of silk fiber, its water vapor absorption properties are reduced by two fold. The optimal time for immobilizing a fibrous polyfunctional hemosorbent based on hydrolyzed fibroin, is 10 minutes.