CHARACTERIZATION AND QUALITY IMPROVEMENT OF MNH992 AND VH289 BT COTTON SEED VARIETIES
SHRINIVASULU KAMMA *
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India
KAVYASRI ARUMILLI
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India
SHAIK MOHAMMAD ANJUM
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India
P. V. HEMANTH SAI
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India
MOHITH PARIMI
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India
VENGILI LAXMISAI DIXITHA
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India
B. VIGNESHWARA REDDY
Department of Humanities and Basic Sciences, Aditya Engineering College, Kakinada, Andhra Pradesh, India
SURYANARAYANA VEERAVILLI
Department of Botany, Yogi Vemanna University, Kadapa, Andhra Pradesh, India
*Author to whom correspondence should be addressed.
Abstract
In this study, we used PCR and ELISA techniques to identify the specific Cry1Ac gene and proteins in MNH992 and VH289 transgenic cotton plants. MNH992 was extracted at a concentration of 21.9ug/g and VH289 at a concentration of 17.37ug/g from seed protein. PCR analysis confirmed that the gene specific CRY1Ac F/R primer produced amplicons with sizes of 550bp for the Bt gene, 600bp for the nptII gene, and 200bp for the 35s promoter gene. The ELISA method was used to validate the developed assay because it required less equipment and took less time.
Keywords: PCR, ELISA, MNH992, VH289, Cry1Ac, Cotton