EFFECT OF MS MEDIUM STRENGTH AND GROWTH REGULATORS (TDZ & KIN) ON Dendrobium nobile ORCHID In vitro REGENERATION

Main Article Content

AZZA M. S. ARAFA
ASMAA B. EL-ATTAR
MONA M. HASSAN
SARA A. EL-SAYED

Abstract

Dendrobium nobile Lind. orchid plants occupy an important role in floriculture trade, as cut flowers, flowering pots and garden plants due to their beautiful attractive flowers with extensive variations in color shoot proliferation.  Trials were carried out for comparing the effects of TDZ and KIN, on Dendrobium in vitro regeneration. In order to achieve the best growth, in vitro-derived plantlets were cultured on full, 3/4 and 1/2 MS salts strengths supplemented with Thidiazuron (TDZ) at 0.0, 1.0, 2.0 and 3.0 mg/l and Kinetin (KIN) at 0.0, 1.0 and 2.0 mg/l in combinations. Healthy individual shoots were cultured on 1/2 MS salts supplemented individually with indole-3-butyric acid (IBA) and 1-naphthalene acetic acid (NAA), at 0, 1, 3 and 10 mg/l to study their effects on root formation. Results in this study, show that full MS was the best strength among MS strengths, gave the best regeneration efficiency, achieving highest values of shoot number, fresh weight and growth value. In addition, different regeneration responses were noticed by using TDZ alone or combined with KIN, the best regeneration efficiency was obtained in a medium supplemented with a combination of KIN and TDZ both at 1.0 mg/l as, it enhanced significantly shoot number and leaf number after 3 subcultures. Furthermore, IBA exceeded NAA in formation of roots (number and length) and shoot fresh weight. Moreover, the plantlets were hardened well in peat moss substrate and achieved 91% of survival rate.

Keywords:
Dendrobium nobile, MS medium strength, TDZ, IBA, NAA, in-vitro

Article Details

How to Cite
ARAFA, A. M. S., EL-ATTAR, A. B., HASSAN, M. M., & EL-SAYED, S. A. (2021). EFFECT OF MS MEDIUM STRENGTH AND GROWTH REGULATORS (TDZ & KIN) ON Dendrobium nobile ORCHID In vitro REGENERATION. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 22(65-66), 99-118. Retrieved from https://www.ikprress.org/index.php/PCBMB/article/view/7162
Section
Original Research Article

References

Chew YC, Halim MHA, Abdullah W/MANW, Abdullah JO, Lai KS. Highly efficient proliferation and regeneration of protocorm-like bodies (PLBS) of the threatened orchid, phalaenopsis sbellina. Sains Malaysiana. 2018;47(6):1093-1099. Available:https://doi.org/10.17576/jsm-2018-4706-03.

Sunitibala DY, Neelashree N. Micropropagation of the monopodial orchid, Rhynchostylis retusa (L.). Int. J. of Life Sciences. 2018;6(1):181-186.

Puchooa D. Comparison of Different Culture Media for the In Vitro Culture of Dendrobium (Orchidaceae). Int. J. Agric. Biol. 2004;6:884-888.

Kongsombut K, Chaisricharoen R. Probabilistic Growth Model for Dendrobium Orchid. Asia-Pacific Signal and Information Processing Association Annual Summit and Conference, 978-616-361-823-8 © 2014 APSIPA; 2014.

Pant B, Thapa D. In vitro mass propagation of an epiphytic orchid, Dendrobium primulinum Lindl. through shoot tip culture. African Journal of Biotechnology. 2016;11(42):9970-9974.

Faria RT, Takahashi LSA, Lone AB, Souza GRB, Silva GL, Hoshino RT. UEL 8: nova cultivar de Dendrobium. Horticultura Brasileira. 2013;31(3):509-511. (Cited after Colombo et al., 2015).

Venturieri GA, Pickscius FJ. Propagação de Olho-de-Boneca (Dendrobium nobile Lindl.) por estaquia. Acta Scientiarum, Agronomy. 2013;35(4),501–504. Available:https://doi.org/10.4025/actasciagro-n.v35i4.15198.

Bhattacharyya P, Kumaria S, Diengdoh R, Tandon P. Genetic stability and phytochemical analysis of the in vitro regenerated plants of Dendrobium nobile Lindl., an endangered medicinal orchid. Meta Gene. 2014;2:489-504. Available:https://doi.org/10.1016/j.mgene.2014.06-.003.

Wang YH. Traditional Uses and Pharmacologically Active Constituents of Dendrobium Plants for Dermatological Disorders: A Review. Natural Products and Bioprospecting. 2021;11(5):465–487. Available:https://doi.org/10.1007/s13659-021-00305-0.

Baker M, Baker CO. Orchid Species Culture. Dendrobium. Portland: Timber Press; 1996.

Hanelt P. IPK-Institut Für Pflanzengenetik und Kulturflanzen. Mansfeld's encyclopedia of agricultural and horticultural crops. Gatersleben: Springer/IPK; 2001.

Vilela XMS, Pasqual M, Villa F, Araújo AG. Tipos de pseudobulbos e número de nós no enraizamento e brotação de Dendrobium nobile. Revista Agrarian. 2010;3(7):1-7. (Cited after Colombo et al., 2015).

Mohanty P, Paul S, Das MC, Kumaria S, Tandon P. A simple and efficient protocol for the mass propagation of Cymbidium mastersii: an ornamental orchid of Northeast India. AoB PLANTS. 2012;1-8. Available:https://doi.org/10.1093/aobp- la/pls023.

Li X, He T, Chun Z. Detecting genetic diversity of Dendrobium tissue culture seedlings with ISSR method. Chin J Appl Environ Biol. 2013a;19:249-254. (in Chinese with English abstract). (Cited after Teixeira da Silva et al., 2015).

Mirani AA, Abul-Soad AA, Markhand GS. Effect of different substrates on survival and growth of transplanted orchids (Dendrobium nobile Cv.) into net house. Int. J. Hortic. Floricult. 2017;5(4):310-317.

Sipayung P, Matanari J, Lafau MB, Sulastri YS, Ginting BB, Sihombing DR, Pandiangan M, Giawa T. The effect of activated charcoal dose and benzyl amino purine concentration on the growth of orchid plantlets in Murashige and Skoog media in vitro. IOP Conference Series: Earth and Environmental Science. 2018;205:1-7. Available:https://doi:10.1088/17551315/2-05/1/012025.

Mangena P. Benzyl adenine in plant tissue culture-succinct analysis of the overall influence in soybean [Glycine max (L.) Merrill.] seed and shoot culture establishment. Journal of Biotech Research. 2020;11(1):23–34.

Wang SY, Steffens GL, Faust M. Breaking bud dormancy in apple with a plant bioregulator, thidiazuron. Phytochemistry. 1986;25:311–317.

Malik KA, Saxena PK. Regeneration in Phaseolus vulgaris L. High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron. Planta. 1992;186:384-389.

Gyulai G, Jekkel Z, Kiss J, Heszky LE. A selective auxin and cytokinin bioassay based on root and shoot formation in vitro. J Plant Physiol. 1995;145:379-382.

Guo B, Abbasi BH, Zeb A, Xu LL, Wei YH. Thidiazuron: A multi-dimensional plant growth regulator. Afr. J. Biotechnol. 2011;10(45):8984-9000. Available:https://doi.org/10.5897/ajb11.636

Sujjaritthurakarn P, Kanchanapoom K. Efficient direct protocorm-like bodies induction of dwarf Dendrobium using thidiazuron. Not Sci Biol. 2011;3:88-92.

Gantait S, Sinniah UR. Rapid micropropagation of monodial orchid hybrid (Aranda Wan Chark Kuan ‘Blue’ x Vanda coerulea Grifft. ex. Lindl. Through direct induction of protocorm-like bodies from leaf segments. Plant Growth Regul. 2012;68:129-40.

Ouyang Y, Chen Y, Lu J, da Silva JAT, Zhang X, Ma G. Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia. Sci Rep. 2016; 6:24662.

Kou Y, Yuan C, Zhao Q, Liu G, Nie J, Zhimin M, Cheng C, da Silva JAT, Zhao L. Thidiazuron triggers morphogenesis in Rosa canina L. protocorm-like bodies by changing incipient cell fate. Front Plant Sci. 2016;7:1-13.

Bhattacharyya P, Kumaria S, Job N, Tandon P. En-masseproduction of eliteclones of Dendrobium crepidatum: A threatened, medical orchid used in Traditional Chinese Medicine (TCM). Journal of Applied Research on Medicinal and Aromatic Plants. 2016;3(4):168-176. Available:https://-doi.org/10.1016/j.jarmap.2016.04.001.

Wu K, Zeng S, da Silva JAT, Chen Z, Zhang J, Yang Y, Duan J. Efficient regeneration of Renanthera Tom Thumb ‘Qilin’ from leaf explants. Sci Hort. 2012; 135:194-201.

Mahendran G, Bai VN. Direct somatic embryogenesis of Malaxis densifolia (A. Rich.) Kuntze. J. Genet Eng. Biotechnol. 2016;14:77-81.

Malabadi RB, Teixeira JA, Mulgund GS. TDZ-Induced in Vitro Shoot Regeneration of Aerides maculosum Lindl. from Shoot Tip Thin Cell Layers. 2009;2(1976):7–11.

Makara M, Rubaihayo PR, Magambo MJS. Carry-over effect of Thidiazuron on banana in vitro proliferation at different culture cycles and light incubation conditions. African Journal of Biotechnology. 2010; 9(21):3079–3085. Available:https://doi.org/10.4314/ajb.v9i21.

Anwar F, Sharmila P, Saradhi PP. No more recalcitrant: Chickpea regeneration and genetic transformation. African Journal of Biotechnology. 2010;9:782–797.

Feng JH, Chen JT. A novel in vitro protocol for inducing direct somatic embryogenesis in Phalaeopsis aphrodite without taking explants. Sci World J. 2014;201:1-7.

Dewir YH, Nurmansyah, Naidoo Y, da Silva TJA. Thidiazuron-induced abnormalities in plant tissue cultures. In Plant Cell Reports. 2018;37(11):1451–1470. Available:https://doi.org/10.1007/s00299-018-2326-1.

Erland LAE, Giebelhaus RT, Victor JMR, Murch SJ, Saxena PK. The morpho-regulatory role of thidiazuron: Metabolomics-guided hypothesis generation for mechanisms of activity. Biomolecules. 2020;10(9):1-34. Available:https://doi.org/10.3390/biom10091253.

Murashige T, Skoog F. A revised medium for rapid growth and bio-assays with tobacco tissue culture. Physiol Plant. 1962; 15:473‒497. DOI: 10.1111/j.1399-3054. 1942.tb 08052.x. (Cited after Mangena, 2020).

Pierik RLM, Dixon RA. In Vitro Culture of Higher Plants. Kluwer Academic Publishers, Dordrecht, The Netherlands; 1997.

Jafari N, Othman RY, Khalid N. Effect of benzyl aminopurine (BAP) pulsing on in vitro shoot multiplication of Musa acuminate (Banana) cv. Berangan. African Journal of Biotechnology. 2011;10(13): 2446-2450. (Cited after Pradhan et al., 2012).

Arditti J. Micropropagation of orchids. 2nd ed. Wiley-Blackwell, Cambridge, UK; 2009.

Ziv M. The use of growth retardants for the regulation and acclimatization of in vitro plants. In: Karssen C.M., van Loon L.C., Vreugdenhil D. (eds) Progress in Plant Growth Regulation. Current Plant Science and Biotechnology in Agriculture. 1992;13. Springer, Dordrecht. Available:https://doi.org/10.1007/978-94-011-2458-4_99 .

Arnon DI. Copper enzymes in isolated chloroplasts polyphenol oxidase in Beta vulgaris. Plant Physiol. 1949;24:1-15.

Lichtenthaler HK. Chlorophylls and Carotenoids, the Pigments of Photosynthetic Biomembranes. In: Douce, R. and Packer, L., Eds., Methods in Enzymology, Academic Press Inc., New York. 1987;350-382.

MSTAT Development Team, MSTAT-C. A microcomputer program for the design, management and analysis of agronomic research experiments, MSTAT Development Team, Michigan State University, East Lansing; 1989.

EISA RST. Studies On Orchid Propagation Through Tissue Culture. Ph.D. Thesis, Depart. of Hort., Fac., of Agric., Ain Shams Univ. 2017;243.

Lakshanthi JMT, Seran TH. Survival rate and growth performance of in vitro raised plantlets of orchid (Dendrobium sp.) in different hardening substrates. IJARR. 2019;4(3):01-9.

Nisayan E, Thepsithar C, Thongpukdea A. Multiple shoot induction from seed derived shoots of paphiopedilum. Acta Horticulturae. 2010;878:199–204. Available:https://doi.org/10.17660/ActaHortic.-2010.878.23.

Tao J, Yu L, Kong F, Zhao D. Effects of plant growth regulators on in vitro propagation of cymbidiumfaberi rolfe. African Journal of Biotechnology. 2011; 10(69):15639–15646. Available:https://doi.org/10.5897/AJB11.1326

Winarto B, Teixeira da Silva JA. Use of Coconut Water and Fertilizer for In Vitro Proliferation and Plantlet Production of Dendrobium ‘Gradita 31.’ In Vitro Cellular and Developmental Biology – Plant. 2015; 51(3):303–314. Available:https://doi.org/10.10-07/s11627-015-9683-z.

Parthibhan S, Rao MV, Kumar TS. In vitro regeneration from protocorms in Dendrobium aqueum Lindley - An imperiled orchid. Journal of Genetic Engineering and Biotechnology. 2015; 13(2):227–233. Available:https://doi.org/10.1016/j.jgeb.2015.07.001.

Murthy BNS, Murch S, Saxena PK. Thidiazuron: a potent regulator of plant morphogenesis. In Vitro Cell Dev Biol Plant. 1998;34:267–275.

Jones MPA, Yi Z, Murch SJ, Saxena PK. Thidiazuron induced regeneration of Echinacea purpurea L.: micropropagation in solid and liquid culture systems. Plant Cell Rep. 2007a;26:13–19.

Lang NT, Hang NT. Omonrice using Biotechnological approaches for Vanda orchid improvement. J Plant Biochem. 2006;14:140-3.

Wattanawikkit P, Bunn E, Chayanarit K, Tantiwiwat S. Effect of cytokinins (BAP and TDZ) and auxin (2,4-D) on growth and development of Paphiopedilum callosum. In Kasetsart Journal - Natural Science. 2011;45(1):12–19.

Hajong S, Kumaria S, Tandon P. Effect of plant growth regulators on regeneration potential of axenic nodal segments of Dendrobium chrysanthum wall. Ex lindl. Journal of Agricultural Science and Technology. 2013;15:1425-1435.

Norhanizan S, Aziz A. Thidiazuron amends the organ development of endangered aquatic plant Cryptocoryne elliptica Hook. F. In IOP Conference Series: Materials Science and Engineering. 2018;440(1). Available:https://doi.org/10.1088/1757-899X/440/1/012046.

Pai SR, Desai NS. Effect of TDZ on various plant cultures. In Thidiazuron: From Urea Derivative to Plant Growth Regulator. 2018;439-454. Available:https://doi.org/10.1007/978-981-10-8004-3_25.

Zahara M, Datta A, Boonkorkaew P, Mishra A. Effect of plant growth regulators on the growth and direct shoot formation from leaf explants of the hybrid Phalaenopsis “Pink.” In Acta Agriculturae Slovenica. 2018;111(1):5–16. Available:https://doi.org/10.14720/aas.2018.111.1.01.

Huetteman C, Preece J. Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult. 1993; 33:105–119. Available:https://doi.org/10.1007/BF01983223.

Thirukkumaran G, Ntui VO, Khan RS, Mii M. Thidiazuron: An efficient plant growth regulator for enhancing Agrobacterium-mediated transformation in Petunia hybrida. Plant Cell Tiss Organ Cult. 2009;99. Available:https://doi.org/10.1007/s11240-009-9581-3.

Nikolić R, Miti N, Mileti R, Nešković M. Effects of cytokinins on In vitro seed germination and early seedling morphogenesis in Lotus corniculatus L. Journal of Plant Growth Regulation. 2006; 25:187-194.

Peres LEP, Majerowicz N, Kerbauy EGB. Dry matter partitioning differences between shoots and roots in two contrasting genotypes of orchids and their relationship with endogenous levels of auxins, cytokinins and abscisic acid. R. Bras. Fisiol. Veg. 2001;13:185-195.

George EF, Hall MA, Deklerk GJ. Plant propagation by tissue culture. Springer. 2008;1:206-217.

Asghar S, Ahmad T, Hafiz IA, Yaseen M. In vitro propagation of orchid (Dendrobium nobile var. Emma white). African Journal of Biotechnology. 2011;10(16):3097–3103. Available:https://doi.org/1-0.5897/ajb10.401.

Nayak NR, Rath SP, Patnaik S. In vitro propagation of three epiphytic orchids, Cymbidium aloifolium (L.) Sw., Dendrobium aphyllum (Roxb.) Fisch and Dendrobium moschatum (Buch. -Ham.) Sw. through thidiazuron-induced high frequency shoot proliferation. Sci. Hort. 1997;71:243-250.

Fukaki H, Tasaka M. Hormone interactions during lateral root formation. Plant Molecular Biology. 2008;69(4):437-49. Available:https://doi.org/10.1007/s11103-008-9417-2.

Husen A, Pal M. Effect of branch position and auxin treatment on clonal propagation of Tectona grandis Linn. F. New Forests. 2007;34:223–233.

Henrique A, Campinhos EN, Ono EO, Pinho SZ. Effect of plant growth regulators in the rooting of Pinus cuttings. Braz Arch Biol Technol. 2006;49:189–196.

Han H, Zhang S, Sun X. A review on the molecular mechanism of plants rooting modulated by auxin. African J Biotech. 2009;8(3):348-353.

Liu C, Zhu J, Liu Z, Li L, Pan R, Jin L. Exogenous auxin effects on growth and phenotype of normal and hairy roots of Pueraria lobata (Willd.) Ohwi. Plant Growth Regulation. 2002;38:37-43. Available:https://doi.org/10.1023/A:1020904528045.

Vuylsteker C, Leleu O, Rambour S. Influence of BAP and NAA on the expression of nitrate reductase in excised chicory roots. J. Exp. Bot. 1997;48:1079-1085.

Rout GR. Effect of auxins on adventitious root development from single node cuttings of Camellia sinensis (L.) kuntze and associated biochemical changes. Plant Growth Regul. 2006;48:111-117.

Khatun H, Khatun MM, Biswas MS, Kabir MR, AL-Amin M. In vitro growth and development of Dendrobium hybrid orchid. Bangladesh J. Agril. 2010;35(3):507–514.

Mirani AA, Abul-Soad AA, Markhand GS. In vitro rooting of Dendrobium nobile Orchid: Multiple responses to auxin combinations. Notulae Scientia Biologicae. 2017;9(1):84–88.DOI: DOI: 10.15835/nsb919894.

Bing HYN, Tham DT, Vinh TKT, Hoi QV, Cong VK, Duy NV. In vitro propagation of the new orchid Dendrobium trankimianum T. Yukawa. Vietnam Journal of Biotechnology. 2020;16(4):649–657. Available:https://doi.org/10.15625/18114989/16/4/1-1155.

Islam T, Bhattacharjee B, Islam SMS, Uddain J, Subramaniam S. Axenic seed culture and in vitro mass propagation of malaysian wild orchid Cymbidium finlaysonianum Lindl. Pak. J. Bot. 2015; 47(6):2361-2367.

Bhandari S, Bisht S, Bisht NS. In vitro propagation in Malaxisacuminata D. Don using pseudobulb explants. Bio-Technology: An Indian Journal. 2011; 5(5):286–289.

Nongdam P, Tikendra L. Establishment of an efficient in vitro regeneration protocol for rapid and mass propagation of Dendrobium chrysotoxum Lindl. Using seed culture. Scientific World Journal. 2014;1-8. Available:https://doi.org/10.1155/2014/740150 .

Riva SS. In Vitro Regeneration and Rapid Multiplication of Two Orchid Varieties of Dendrobium bensoniae and Dendrobium aphyllum. Biotechnology Program, Department of Mathematics and Natural Sciences BRAC, University Bangladesh. 2016;1–67.

Patil JG, Ahire ML, Nikam TJ. Influence of plant growth regulators on In vitro seed germination and seedlings development of Digitalis purpurea L. Asian and Australasian Journal of Plant Science and Biotechnology. 2012;6:12-18.

Anuchai J, Sasiangdee A. Effect of growth regulator on shoot induction from protocorm of Dendrobium anna. International Journal of Agricultural Technology. 2020;16(6):1319–1330.