SEQUENCE-RELATED AMPLIFIED POLYMORPHISM (SRAP) MARKERS FOR GENETIC VARIATION AMONG Capsicum frutescens L. POPULATIONS FROM KANCHANABURI PROVINCE, THAILAND
NANTHAWAN HADTHAMARD
Faculty of Science and Technology, Kanchanaburi Rajabhat University, Kanchanaburi 71190, Thailand.
NITTAYA WANIKORN
Faculty of Science and Technology, Kanchanaburi Rajabhat University, Kanchanaburi 71190, Thailand.
MUKDA AIAMCHAROEN
Khao Jod Organic Farming Community Enterprise, Kanchanaburi 71220, Thailand.
MALIWAN NAKKUNTOD *
Department of Biology, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand.
*Author to whom correspondence should be addressed.
Abstract
Capsicum frutescens L. is one of the most important species of the genus Capsicum, and widely distributed in the world. Based on its high benefits, chili pepper has become one of the horticultural commodities that highly cultivated by Thai-Karen people in Thailand. The efficiency and accuracy in genetic diversity are an importance for postharvest utilization of Capsicum. In this study sequence-related amplified polymorphism (SRAP) marker was analyzed the genetic diversity among 22 C. frutescens cultivated from 7 sub-districts in Kanchanaburi province. Out of 100 combination primers, 4 combination primers detected a total of 55 alleles (average, 13.75; ranged 9-18). The average polymorphic information content (PIC) was 0.30 (ranged 0.28–0.32). Among C. frutescens populations, the UPGMA dendrograms based on SRAP markers could generate and divide into three main clusters. The C. frutescens populations cultivated in Khao Chot sub-district, Si Sawat district in Kanchanaburi were grouped within all three main clusters. This result indicated that SRAP molecular markers are useful for studying the genetic diversity among populations of C. frutescens. This information can approve the breeding program and postharvest potential utilization in the further.
Keywords: Capsicum frutescens L., SRAP, genetic diversity