LARVICIDAL ACTIVITY OF EXTRACTS FROM SIX PLANT SPECIES ON LARVAE OF Culiseta longiareolata (DIPTERA; CULICIDAE)
NORA BELKHIRI
Laboratory for Improving Agricultural Production and Protection of Resources in Arid Zones, Institute of Veterinary and Agronomic Sciences, University of Batna 1, 5000, Algeria
SALIHA BENHISSEN *
Applied Neuroendocrinology Laboratory, Department of Biology, Faculty of Sciences, BP 12 Badji Mokhtar University, 23000 Annaba, Algeria and Department of Natural and Life Sciences, Faculty of Sciences, Mohamed Boudiaf University of M'sila, 28 000, Algeria.
WAFA HABBACHI
Applied Neuroendocrinology Laboratory, Department of Biology, Faculty of Sciences, BP 12 Badji Mokhtar University, 23000 Annaba, Algeria
ABDELMADJID YAGOUB ASLOUM
Ecology of Terrestrial and Aquatic Systems, Department of Biology, Faculty of Sciences, University of Badji Mokhtar, Annaba, Algeria
ZAKARIA HEDJOULI
Applied Neuroendocrinology Laboratory, Department of Biology, Faculty of Sciences, BP 12 Badji Mokhtar University, 23000 Annaba, Algeria
SARRA HABBACHI
Department of Natural and Life Sciences, Faculty of Sciences, Mohamed Boudiaf University of M'sila, 28 000, Algeria
KHELLAF REBBAS
Department of Natural and Life Sciences, Faculty of Sciences, Mohamed Boudiaf University of M'sila, 28 000, Algeria and Laboratory of Agro-Biotechnology and Nutrition in Arid and Semi-Arid Zones/ Natural Resources Management and Environment Research Team, University Ibn Khaldun, Tiaret, Algeria.
NAAMA FRAH
Laboratory for Improving Agricultural Production and Protection of Resources in Arid Zones, Institute of Veterinary and Agronomic Sciences, University of Batna 1, 5000, Algeria
*Author to whom correspondence should be addressed.
Abstract
Because of the environmental problems and dangers to human health caused by chemical insecticides, the use of natural biocides seems to be imperative. In this context toxicity tests were carried out according to the protocol of the World Health Organization (WHO) for six aqueous extracts of leaves of: Ambrosia Maritima, Hertia centifolia, Xanthium strumarium, Datura stramonium, Solanum elaeagnifolium, and Salvea verbena, with a series of three doses for each extract. The evaluated extracts showed good larvicidal activity against the fourth instar larvae of Culiseta longiareolata mosquito, the decoction method is used for the preparation of extracts. The mortality rate increases depending on the concentration of the used extract and the exposure time, with interesting lethal concentrations LC50% and LC90%, A. Maritima (11.65 μg / ml and 52.40 μg / ml) after 120 hours and D. stramonium (16.94 μg / ml and 28.36 μg / ml) after 96 hours. While the lethal times LT50% and LT90% do not exceed (0,01 day to 2,14 day) at a dose of 219.9 μg / ml of S. elaeagnifolium (0,95 day to 1,34 day) with a dose of 160 μg / ml of H. centifolia.
s. elaeagnifolium, H. centifolia and D. stramonium showed an excellent larvicidal activity of the aqueous extract of the leaves of the studied plants.
Keywords: Biological activity, Solanum elaeagnifolium, Hertia centifolia, Datura stramonium, Culiseta longiareolata, lethal concentrations LC50%, lethal times LT50%