In vitro ANTIOXIDANT ACTIVITY OF Hypnea Pannosa J.AGARDH

Main Article Content

R. RABECCA
A. DOSS

Abstract

The goal of this study was to investigate the antioxidant properties of crude extracts of Hypnea pannosa, a red alga from the Hypneaceae family that was collected from the Gulf of  Manner in Rameswaram, Tamilnadu. The extracts were assayed for DPPH radical scavenging, Hydroxyl radical, ABTS radical, Superoxide radical, and reducing power. Ethanol and methanol extracts were found to exhibit significant antioxidant activity. Ethanol extract of H. pannosa showed a good significant activity for ABTS and DPPH free radicals (118.56% and 124.30%). This holds great promise for the use of Hypnea pannosa as a source of strong antioxidant compounds.

Keywords:
Red algae, ABTS, DPPH, reducing power, free radical activity

Article Details

How to Cite
RABECCA, R., & DOSS, A. (2021). In vitro ANTIOXIDANT ACTIVITY OF Hypnea Pannosa J.AGARDH. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 22(51-52), 95-103. Retrieved from https://www.ikprress.org/index.php/PCBMB/article/view/6993
Section
Original Research Article

References

Farah Mazhar, Mohtasheemul Hasan, Iqbal Azhar, M. Shaiq Ali, Misbah Zubair, Rabail Zahid and Muhammad Akram. Some biological studies on Hypnea pannosa J. Ag. Afr J Biotechnol. 2011; 10(61):13313-13317.

Andrade KAM, Chiara Lauritano ID, Giovanna Romano, Adrianna Ianora. Marine microalgae with anti-cancer properties. Mar Drugs. 2018;16:165. DOI: 10.3390/md16050165.

Perez MJ, Elena Falque, Herminia Domínguez. Antimicrobial action of compounds from Marine Seaweed. Mar Drugs. 2016;14:52. doi:10.3390/md14030052.

Cornish ML, Garbary DJ. Antioxidants from macroalgae: Potential applications in human health and nutrition. Algae. 2010;25:155–171.

Devi KP, Natarajan Suganthy, Periyanaina Kesika and Shanmugaiahthevar Karutha Pandian. Bioprotective properties of seaweeds: In vitro evaluation of antioxidant activity and antimicrobial activity against food borne bacteria in relation to polyphenolic content. BMC Compl Alter Med. 2008;8:38.

Hwang PA, Chwen-Herng Wu, Shu-Yun Gau, Shih-Yung Chien and Deng-Fwu Hwang, Antioxidant and immune-stimulating activities of hot-water extract from seaweed Sargassum hemiphyllum. J Marine Sci Technol. 2010;18(1):41-46.

Siddique MAM, Aktar M, Khatih MAM. Proximate chemical composition and amino acid profile of two red seaweeds (Hypnea pannosa and Hypnea musciformis) collected from St. Martin’s Island. Bangaldesh J Fisher Sci. 2013;7(2):178-186.

Afaq-Hussain S, Shameel M, Usmanghani K, Ahmad M, Perveen S, Ahmad VU. Brominated sesquterpene metabolites of Hypnea pannosa (Gigartinales, Rhodophyta). J Appl Phycol. 1991; 3(2):111-113.

Shanmugapriya S, Manilal A, Sujith S, Selvin J, Kiran GS, Natarajaseenivasan K. Antimicrobial activity of seaweeds extracts against multi-resistant pathogens. Annals of Microbiol. 2008; 58(3):535-541.

Mazhar F, Hassan M, Azhar I, Ali MS, Zubair M, Zahid R, Akram M. Some biological studies on Hypnea pannosa J. Ag. Afr J Biotechnol. 2011;10(61):13313-13317.

Alam MT, Usmanghani K. Studies on marine algae for haeagglutine activity. Pak J Pharm Sci. 1994;7(2):1-15.

Blois MS. Antioxidant determination by the use of a stable free radical. Nat. 1958;181:1199- 1200.

Halliwell B, Gutteridge JMC, Aruoma OI. The deoxyribose method: a simple 'test tube' assay for determination of rateconstants for reaction of hydroxyl radicals. Anal Biochem. 1987;165:215-219.

Srinivasan R, Chandrasekar MJN, Nanjan MJ, Suresh B. Antioxidant activity of Caesalpinia digyna root. J Ethnopharmacol. 2007;113:284-291.

Huang MH, Huang SS, Wang BS, Wu CH, Sheu MJ, Hou WC, Lin SS, Huang GJ. Antioxidant and antiinflammatory properties of Cardiospermum halicacabum and its reference compounds ex vivo and in vivo. J Ethnopharmacol. 2011;133:743-750.

Kumar RS, Hemalatha S. In vitro antioxidant activity of alcoholic leaf extract and subfractions of Alangium lamarckii Thwaites. J Chem Pharma Res. 2011;3:259-267.

Garcia EJ, Oldoni TLC, Alencar SM de, Reis A, Loguercio AD, Grande RHM. Antioxidant activity by DPPH assay of potential solutions to be applied on bleached teeth. Braz Dent J. 2012;23(1):22–27.

Dore CMPG, das C Faustino Alves MG, Will LSEP, Costa TG., Sabry DA., de Souza Rego LAR., Accardo CM, Rocha HAO, Filgueira LGA, Leite EL. A sulfated polysaccharide, fucans, isolated from brown algae Sargassum vulgare with anticoagulant, antithrombotic, antioxidant and anti-inflammatory effects. Carbohydr Polym. 2013;91(1):467–475.

Ebrahimzadeh ML, Masoumeh Khalili, Abbas Ali Dehpour. Antioxidant activity of ethyl acetate and methanolic extracts of two marine algae, Nannochloropsis oculata and Gracilaria gracilis – an in vitro assay. Braz J Pharm Sci. 2018;54(1):e17280.

Heo SJ, Seon-Heui Cha, Ki-Wan Lee, You-Jin Jeon. Antioxidant activities of red algae from Jeju Island. Algae. 2006;21(1):149-156.

Magalhaes KD, Costa LS, Fidelis GP. Anti-coagulant, Anti-oxidant and Anti-tumor Activities of Heterofucans from the Seaweed Dictyopteris delicatula. Int J Mol Sci. 2011;12:3352-3365.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med. 1999;26(9-10):1231–1237.

Matkowski A, Tasarz P, Szypuła E. Antioxidant activity of herb extracts from five medicinal plants from Lamiaceae, subfamily Lamioideae. J Med Plants Res. 2008;2(1):321–330.

Louis Cojandaraj, Surya Prabha U. and Mary Elizabeth Shyamala. Antioxidant activity of marine red algae – Portieria hornemannii. Plant Archives. 2020; 20(Supplement 2):1075-1081.

Ukeda H, Shimamura T, Tsubouchi M, Harada Y, Nakai Y, Sawamura M. Spectrophotometric assay of superoxide anion formed in Maillard reaction based on highly water-soluble Terazolium salt. Jpn Soc. Anal. Sci. 2002;18:1151-1154.