PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

This study aimed to determine the activity of Trigonella foenum-graecum extracted with different solvents based on their polarity indexes then combined with antibiotics against Methicillin-Resistant Staphylococcus aureus (MRSA). The phytochemical qualitative analysis revealed the presence of alkaloids, phenols, tannins, flavonoids, and saponins. On the evaluation of the antimicrobial susceptibility, MRSA strain was standardized using the colony suspension method. The suspensions matched with 0.5 McFarland standards resulting concentration of 1.5 × 106 CFU/mL. Antimicrobial susceptibility tests were carried on both disc and well diffusion methods on Mueller Hinton agar under the steps on the Kirby-Bauer test. 50 μL of the bacterial sample were inoculated into Mueller Hinton agar plates. Methanol Trigonella foenum-graecum extract revealed a higher antibacterial activity against Methicillin-Resistant Staphylococcus aureus. There was a significant increase in the diameter zone of inhibition on the combination of the methanol plant extract with Ciprofloxacin (5mcg) of 2 ± 0.4 mm. Methanol T. foenum-graecum with Tetracycline (30 mcg) increased by 2 ± 0 mm. Water plant extract with Ciprofloxacin (5mcg) to 1 ± 0.3 mm and T. foenum-graecum with Tetracycline (30 mcg) increased by 1 ± 0.7 mm. Isopropanol plant extract only showed an increase in the diameter zone of inhibition with Ciprofloxacin (5mcg) of 1 ± 0.4 mm. Hexane solvent extract did not show an increase in its diameter zone of inhibition. This study is of great significance in advancing the efficacy of antibiotics to broader-spectrum against pathogenic multidrug-resistant microorganisms.