PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Ethnobotanical and biochemical studies are useful to discover new drugs. Biochemical screening is an important step in the detection of the bioactive components existing in medicinal plants that are used in traditional medicine. Throughout history, human civilizations have kinetically circumvented plants which have influenced a lot to the humanity. Plants have the facility to endanger diverse variety of phytochemical and biochemical compounds which can be acclimated to perform different biological functions. Many of these phytochemicals have salutary effects on long-term health when consumed by the human and can be efficaciously used to treat human diseases. Alternative medicine has become popular these days as it is gaining practices across the globe. The present study is to focus on the determination of effective combination of plant based extracts for antioxidant property by adopting bio guided assisted methods. Three medicinal plants commonly used in traditional and herbal formulations for various types of ailments were selected based on their efficacy on diabetes mellitus (DM). They are the fruits, bark of Aegele marmelos, fruit of Musa paradisiaca and aerial parts of Ocimum sanctum. All the samples were subjected to biochemical analysis and in vitro anti-oxidant activity by DPPH radical scavenging. The anti-oxidant activity of the bio guided combination of the extracts enables us to derive the effective polyherbal combination for the activity. The antioxidant activity was found to be maximum of the methanolic extracts of all three plants emphasizing that methanol has been the best solvent for extraction. Moreover due the presence of phenolic compounds in these plants they exhibited antioxidant activity and thus are beneficial in the treatment and management of Diabetes mellitus.