IMPACT OF ANTIOXIDANTS AND COBALT ON GROWTH PERFORMANCE OF BROCCOLI PLANTS IRRIGATED BY DIFFERENT IRRIGATION SOURCES

Main Article Content

KARIM F. FOUDA

Abstract

Use of different irrigation sources e.g, agricultural drainage water becomes necessary to mitigate the expected water scarcity in the future. So, a field trial was implemented in a split split-plot design with three replicates aiming at evaluating the impact of three sources of irrigation water as main plots [normal irrigation water (freshwater), agricultural drainage water and a mixture between them], foliar application of two antioxidants [ascorbic acid and proline at a rate of 10.0 mM L-1  for both alone and volume of 500 L ha-1] as subplots and foliar application of cobalt as sub subplots [0.0 and 8.0 mg cobalt L-1 as cobalt sulphate solution (36%Co) at volume of 500 L ha-1] on the performance of broccoli plants. The obtained results showed that broccoli plants irrigated by normal water have a high performance and yield, while broccoli plants irrigated by mixture water came in the second order, whilst the lowest was realized with broccoli plants irrigated with agricultural drainage water alone. Foliar application of ascorbic acid was the superior treatment followed by proline and lately control treatment (without antioxidants).Also, foliar application of cobalt at rate of 8.0 mg cobalt L-1 came in the first order, while the second order went to plants untreated with cobalt (0.0 mg cobalt L-1). Generally the best performance and yield were recorded when broccoli plants were irrigated by normal irrigation water (fresh water) and sprayed with both ascorbic acid and cobalt. Taking into account that spraying broccoli plants with both antioxidants (ascorbic acid or proline) and cobalt together under irrigation by   mixture water possessed  better performance and yield than broccoli plants irrigated  by normal  water without  foliar any application.

Keywords:
Agricultural drainage water, ascorbic acid, proline, cobalt and broccoli plants.

Article Details

How to Cite
FOUDA, K. F. (2021). IMPACT OF ANTIOXIDANTS AND COBALT ON GROWTH PERFORMANCE OF BROCCOLI PLANTS IRRIGATED BY DIFFERENT IRRIGATION SOURCES. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 22(41-42), 24-32. Retrieved from https://www.ikprress.org/index.php/PCBMB/article/view/6650
Section
Original Research Article

References

El-Hadidi EM, Meleha AMI, El-Tobgy SMM, El-Ezz SA. Response of rice (Oriza sativa L.) to some antitranspiratiors under water stress in north Nile Delta, Egypt. Plant Archives. 2020;20(2):2210-2220.‏

Ozgur R, Uzilday B, Sekmen AH, Turkan I. Reactive oxygen species regulation and antioxidant defence in halophytes. Functional Plant Biology. 2013;40:832–847.

Osman AS, Wahed MHA, Rady MM. Ascorbic acid improves productivity, physio–biochemical attributes and antioxidant activity of deficit-irrigated broccoli Plants. Biomedical Journal. 2018;1:10.‏

Gest N, Gautier H, Stevens R. Ascorbate as seen through plant evolution: the rise of a successful molecule. Journal of Experimental Botany. 2013;64:33– 53.

Conklin PL. Recent advances in the role and biosynthesis of ascorbic acid in plants. Plant Cell Environ. 2001;24:383–394.

Butt M, Ayyub CM, Amjad M, Ahmad R. Proline application enhances growth of chilli by improving physiological and biochemical attributes under salt stress. Pakistan Journal of Agricultural Sciences. 2016;53(1).

Official Journal of the European Union. Regulation (Eu) 2019/1009 of the European Parliament and of the Council of 5 June 2019 Laying down Rules on the Making Available on the Market of EU Fertilising Products and Amending Regulations (EC) No 1069/2009 and (EC) No 1107/2009 and Repealing Regulation (EC) No 2003/2003. L 170. 25 June 2019;62.
Available: https://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=OJ:L:2019:170:FULL&from=EN
(accessed on 22 January 2020)

Akeel A, Jahan A. Role of cobalt in plants: its stress and alleviation. In Contaminants in Agriculture. Springer, Cham.‏ 2020;339-357.

Shanon MC. Effect of cobalt on tomato plants grown under salinity conditions. J. Amer. Soc. Hort. Sci. 2002;12:644‏‏.

Hossain M, Mohona MJ. Shoot-root traits of broccoli (Brassica oleracea var. italica L.) as influenced by different irrigation schedules. International Journal of Horticultural Science and Technology. 2018;5(1): 11-18.‏

Buurman P, Van Lagen B, Velthorst EJ. Manual for soil and water analysis. Backhuys; 1996.

Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water stress studies. Plant and Soil. 1973;39:205-208.

Eberhardt MV, Lee CY, Liu RH. Antioxidant activity of fresh apples. Nature. 2000;405:903–904.

Śmiechowska A, Bartoszek A, Namieśnik J. Determination of glucosinolates and their decomposition products—indoles and isothiocyanates in cruciferous vegetables. Critical Reviews in Analytical Chemistry. 2010;40(3):202-216.

A.O.A.C. Association of official agriculture chemistry. Official Methods; 1980.

Nerdy N, Putra EDL. Spectrophotometric method for determination of nitrite and nitrate levels in broccoli and cauliflower with different fertilization treatment. Oriental Journal of Chemistry. 2018;34(6): 2983.‏

Duncan DB. Multiple range and multiple Ftest. Biometrics. 1955;11:1-42.

Guo L, Yang R, Wang Z, Guo Q, Gu Z. Effect of NaCl stress on health-promoting compounds and antioxidant activity in the sprouts of three broccoli cultivars. International Journal of Food Sciences and Nutrition. 2014;65(4):476-481.‏

Zaghdoud C, Bagues M, Yahia Y, Nagaz K. Endogenous minerals conditioned genotypic variation of phytochemicals in broccoli leaves under salinity stress. Polish Journal of Environmental Studies. 2021; 30(3):2381–2394.

El-Beltagi HS, Mohamed HI, Sofy MR. Role of ascorbic acid, glutathione and proline applied as singly or in sequence combination in improving chickpea plant through physiological change and antioxidant defense under different levels of irrigation intervals. Molecules. 2020;25(7):1702.‏

Rathsooriya GB, Nagarajah S. Studies effect of salinity with cobalt on tomato plants. Plant and Soil. 2003;44: 158.

Gad N, Abd El-Moez MR, Kandil H. Barley response to salt stress at varied levels of cobalt II. Some physiological and chemical characteristics. Journal of Applied Sciences Research. 2011;7:1447-1453.