PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

The banana tissue culture plants are gaining importanceamong farmers due to free of pests and diseasesbanana plantlets and high yielding variety. Therefore, there has been widespread use of plant tissue culture in micropropagation of banana plants as planting material.The success of farmers for producing export quality banana plants with productivity is due to mass propagation of tissue culture banana plantlets and its economic viability however, depends upon several factors which influence the scaling-up as Banana Tissue Culture plants Industry.Once the mass production at laboratory it is important to survive the plants upto field planting. Improper hardened plants show poor vigor of plants and root system which results to many losses after field planting. As per experience during hardening and acclimatization the major role of plants’ survival with help of perfect recipe of growing media and high tech GreenHouses with full automation for primary hardening and Polyhouse for secondary hardening because of this tissue culture technology to large-scale propagation, it is necessary to develop hardening methods that are relatively simple and have a high survival rate in Farmers’ field. Tissue culture plantlets upon transfer to ex-vivoconditions, innovative approaches such as the use of different colour shade-nets and growing media in ex-vivo hardening, within a short period and improvement in growth without losses and perfect monitoring of off type plants.