In-vitro EVALUATION OF ANTI-OXIDANT AND ANTI-DIABETIC ACTIVITY OF Calotropis procera
ROHIT THAKKAR
School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab -144411, India
RAJAN KUMAR
School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab -144411, India.
AMRIT PAL SINGH
School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab -144411, India.
MANISH VYAS *
School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab -144411, India.
NAVNEET KHURANA
School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab -144411, India.
NEHA SHARMA
School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab -144411, India.
DHARAM BUDDHI
Division of Research and Development, Lovely Professional University, Phagwara, Punjab -144411, India.
PRAMOD YADAV
Department of Rasashastra & Bhaishajya Kalpana, All India Institute of Ayurveda, New Delhi-110076, India.
P. K. PRAJAPATI
Department of Rasashastra & Bhaishajya Kalpana, All India Institute of Ayurveda, New Delhi-110076, India.
BHUPENDRA TOMAR
Abhilashi College of Pharmacy, Ner Chowk, Mandi, Himachal Pradesh – 175008, India.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Herbal plants are known for their anti-oxidant and other various therapeutic activities. Mostly the active chemical moieties of the herbal plants are flavonoids in nature. In the present study, Calotropis procera was evaluated for its anti-oxidant and anti-diabetic activity.
Materials and Methods: First the ethanolic extract of leaves of the plant was prepared and then subjected to the in-vitro anti-oxidant and anti-diabetic evaluation. Anti-oxidant activity was evaluated by using DPPH and hydrogen peroxide assay whereas Alpha amylase assay was performed to evaluate the anti-diabetic activity. The result of the extract was also compared with the standard compound.
Results and Discussion: The IC50 value for extract was found to be less when compared with the standard which represents that extract have good anti-oxidant and anti-diabetic activity.
Keywords: Anti-oxidant, diabetes mellitus, alpha amylase, free radicals