POTENTIAL OF HORMONES COMBINATION ON CALLUGENSIS OF ANISE SEEDS AND ITS ANTIOXIDANT ACTIVITY
MANEEA MOUBARAK
Department of Horticulture, Faculty of Agriculture, Damanhour University, Egypt.
YASIR S. SEKHI
Department of Horticulture, College of Agriculture, University Of Anbar, Iraq.
AMMAR AL-FURAIJI
Department of Accreditation and Quality Assurance, Ministry of Higher Education and Scientific Research, Baghdad, Iraq.
ALI F. ALMEHEMDI
*
Department of Conservative Agriculture, Center of Desert Studies, University Of Anbar, Iraq.
AHMED A. ALMARIE
Department of Field Crops, College of Agriculture, University Of Anbar, Iraq.
*Author to whom correspondence should be addressed.
Abstract
In vitro, a culture experiment was initiated from the seeds of Pimpinella anisum L. to investigate callugensis (regeneration and rooting) and antioxidant formation. Three hormones with three different combinations were used in this experiment; Benzylaminopurine (BAP) in three concentrations (0.5, 1, 1.5, and 2 mg L-1) alone and combinations with 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5, 1, 1.5, and 2 mg L-1), or combination with indole butyric acid (IBA) (1 and 1.5 mg L-1). A completely randomized design (CRD) was used with three replicates followed by Fisher’s LSD test (P < 0.05). The results revealed that Murashige and Skoog (MS) medium fortified by (0.5 mg L-1BAP and 1 mg L-1 2,4-D) was significantly the best combination for improving the fresh weight of 23.66 g and total phenolics of 536.5 mg. Furthermore, a combination of 1.5 mg L-1 BAP and 1.5 mg L-1IBA gave the best dry weight of 1.8267 g. While applied (2 mg L-1 BAP and 1.5 mg L-1 IBA increases 2,2-diphenyl 1-picrylhydrazyl (DPPH) estimation method by 71.06%. It was concluded that binary combinations were efficient to maximize phenolics resulted in augmenting antioxidant potentiality.
Keywords: Anise, Callus, Phenolic, Benzyl amino purine, Indole butyric acid