PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Indonesia needs to be encouraged by the discovery of new bioinsecticides substituting synthetic insecticides, which have posed a danger to human survival. One of the plants that have the potential to become bioinsecticides is kaffir lime (Citrus hystrix DC). On the skin’s kaffir lime contains chemical compounds such as secondary metabolites and essential oils. The most considerable and largest content of kaffir lime essential oil is limonene or limonoid. This compound is a member of monoterpenes which has vigorous insecticide activity. Limonene/limonoid act as stomach poisons of mosquito larvae. Limonene/limonoid enters the body of the larvae through immersion of the consumed extract concentration. This research aims to determine the right formulation and specifications through physical properties test and determine the lethal concentration 95 ( ) of kaffir lime granule extract (Citrus hystrix DC) against Aedes aegypti mosquito larvae so that it meets the requirements as a new bioinsecticide. Physical tests carried out include tests of water content, flow time test, moisture content test, indexing, and fragility test. Based on the results of formulations and specifications through physical properties test, the water content of granules is 0.80-1.40%, the flow time is 7.00, the moisture content is 3.40, the index is 7.11, and the fragility is 0.2. While the results of probit analysis obtained the value of  of kaffir lime skin extracts (Citrus hystrix DC.) for 24 hours of bran is 7915.85 ppm with a lower limit of 6802.33 ppm and an upper limit of 9718.21 ppm.