PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

The research was conducted in the year 2019-2020 at the Field Crops Department / College of Agriculture and Forestry / University of Mosul to study variation of resistance of Mung bean to salinity in response to by using some growth regulators in terms of mineral and proline content in leaves and protein content in beans and leaves. The experiment was carried out in pots on in two season, using the green local variety called Green gram (Vigna radiata L.) obtained from the Dohuk Agricultural Department and the experiment included two factors; growth regulators each of (control, (gibberellic acid (GA3), kinetin (Kin), salicylic acid (SA) or a combination of two regulators at a concentration of 100 mg.L^-1 per treatment while the second factor include ; sodium chloride salt (NaCl) with three concentrations (0.03, 5.0, and 10.0) dS.m^-1 alone and in combination with growth regulators. A completely randomized design (CRD) was used Duncan test was used to determine the differences between treatments. Results of the study showed that all growth regulators (except for Kin) stimulated the significant increase in the content of nitrogen element( N) content in leaves at in control plants and under in leaves at (0.0 and 5 dS.m^-1) at the first date. On the second date, it was noticed that SA and GA₃ significantly superior at the level of 5 dSm^-1, and all growth regulators superior at the level of 10 dS.m^-1.

Phosphorous (P) was significantly increased by GA₃ and Kin in control plants on date, and at the second second date, GA₃ and (SA + Kin) increased  P content under saline conditions  at 5 and 10 dS.m^-1.

As for potassium (K) in the leaves, it was significantly increased by all growth regulator treatments in the absence of salt stress, while under salt stress Kin, SA, and (SA + GA₃) increased the P content at the first date, and  GA₃ and SA at the second date at  5 dS.m^-1.

On the other hand, all growth regulators caused a decrease in the content of NaCl a tall salinity levels and dates. It was also observed that all growth regulators caused a significant increase in the content of proline on the first and second dates. The intensity of the effect increased with increasing salinity, and the SA and its interactions were characterized at the highest levels.

All growth regulators significantly increased the content of chlorophyll, protein in seeds, and protein in leaves in the first and second dates even under salinity levels. SA was characterized by the highest levels.