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Nothapodytes nimmoniana (Graham) Mabb. (N. nimmoniana), gaining more attention for its wide range of medicinal uses and its pharmaceutical activities. The present study was designed to standardize the protocol for embryo culture, callus induction and its phytochemical and antioxidant activities. Mature embryos of N. nimmoniana were inoculated on L2 medium supplemented with cytokinins (2 mg/L of BAP and 1 mg/L of KIN). Callus induction was observed from nodal explants derived from regenerated shoots, when sub cultured on L2 medium fortified with BAP (0.5 mg/L) + NAA (1 mg/L) + 2,4-D (1 mg/L). Preliminary screening reveals the presence of various primary and secondary phytochemicals like alkaloids, flavonoids, polyphenols, steroids, saponins, tannins in leaf, bark, root, callus and in vitro root. Total alkaloids content (57.40 ± 0.50 µg of CE /mg) was found to be higher in methanolic extract of root. Whereas leaf extract revealed higher content of total flavonoids (87.91 ± 0.65 µg of QE /mg) and total tannins (35.36 ± 0.095 µg of TAE/mg). The total phenolics were found to be maximum in the callus extract (55.58 ± 0.28 µg of GAE/mg). Root extracts showed significant DPPH and ABTS scavenging activity with an IC50 values of 51.52 ± 0.40 µg/mL and 26.64 ± 0.36 µg/mL respectively. Leaf extract demonstrated better total antioxidant and reducing power activity with an IC50 value of 70.63 ± 0.38 µg of AAE/mg and 396.33 ± 1.53 µg/mL. Our study demonstrated that methanolic extract proves to be a better solvent for extraction of phytochemicals and antioxidant activity and further studies are required to isolate the bioactive compounds from the crude extract.

Nothapodytes nimmoniana, Icacinaceae, callus, phytochemicals, antioxidant

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Gowda HC, Vasudeva R, Georgi PM, Uma SR, Ganeshaiah KN. Breeding types in Nothapodytes nimmoniana Graham. Current Science. 2002;83:1077-78.

Wall ME, Wani MC. CPT and Analogs: From discovery to clinic. CRC Press: Boca Raton. 1995;21-41.

Panneerselvam K, Bhavanisankar K, Jayapragasam K. Effect of growth regulators and planting media on rooting of cuttings of N. nimmoniana Mabberly. Indian Journal of Plant Physiology. 2004;9:308–312.

Redinbo MR, Styewart Lance, Kuhn Peter, Champoux James J, Hol Wim GJ. Crystal structures of human topoisomerase I in covalent and monocovalent complexes with DNA. Science. 1998;279:1504-13.

Raveendran VV. Camptothecin-discovery, clinical perspectives and biotechnology. Nat Prod Chemical Research. 2015;3(175):2.

Ved DK. Trend in medicinal plants. The state of our ignorance. Amruth. 1997;1:2-8.

Padmanabha BV, Chandrashekar M, Ramesha BT, Hombe Gowda HC, Gunaga RP, Suhas S. Pattern of accumulation of Camptothecin, an anticancer alkaloid in Nothapodytes nimmoniana Graham. In the Western Ghats, India: Implications for identifying high yielding sources of alkaloid. Current Science. 2016;90(1):95–99.

Suhas S, Ramesh BT, Ravikanth G, Rajesh P Gunaga, Vasudeva R, Ganeshaiah KN, Uma Shanker. Chemical profiling of Nothapodytes nimmoniana in the Western ghats, India for anti-cancer compound, Camptothecin. Current Science. 2007;92:1142-47.

Namdeo AG, Sharma A, Mahadik KR. Antioxidant activity of methanolic extracts of Nothapodytes nimmoniana (J. Graham) Mabberly. Pharmacology online. 2010;1:148‐59.

Takeuchi A, Dohashi K, Fujimoto S, Tanaka K, Suzuki M, Terashima Y, Hasumi K, Akiya K, Negishi Y, Tamaya T. A late phase II study of CPT-II in uterine, cervical cancer and ovarian cancer. Japan Journal of Cancer Chemotherapy. 1991;18:1661–89.

Bodley AL, Shapiro TA. Molecular and cytotoxic effects of Camptothecin, a Topoisomerase I inhibitor, on Trypanosomes and Leishmania. Proceedings of the National Academy of Sciences of the USA. 1995;92:3726-30.

Dharmalingam SR, Madhappan R, Ramamurthy S, Meka VS, Shanmugham S, KL SK. Anticarcinogenic effects of Nothapodytes nimmoniana against Dalton’s lymphoma as cites tumor model. European journal. Experimental Biology. 2014;4(1):527-533.

Sharma S, Kumar A, Namdeo A. Pharmacognostical and phytochemical analysis of Nothapodytes nimmoniana stem. International Journal of Pharmacy and Pharmaceutical Science. 2012;4(4):455-459.

Phillips GC, Collins GB. Virus symptom-free plants of red clover using meristem culture. Crop Science. 1979;19:213- 221.

Saxena M, Saxena J, Nema R, Singh D, Gupta A. Phytochemistry of medicinal plants. Journal of Pharmacognosy and Phytochemistry. 2013;1(6):168-182.

Sofowora A. Medicinal plants and traditional medicine in Africa. Spectrum Books Ltd (Pub.), Ibadan; 1993.

Trease GE, Evans WC. Pharmacognosy. Bailliere Tindall, London. 1989;45-50.

Kokate CK. Practical Pharmacognosy. Vallabh Prakashan, New Delhi. 1994;1:15-30.

Harborne JB. Phytochemical methods. A guide to Modern Technique of Plant Analysis, 2nd ed. Chapman. 1984;100-101.

Raman N. Phytochemical technique. New Indian Publishing Agencies, New Delhi; 2006.

Singh DK, Srivastava B, Sahu A. Spectrophotometric determination of Rauwolfia alkaloids: estimation of reserpine in pharmaceuticals. Analytical Science. 2004;20(3):571-573.

Singleton VL, Orthofer R, Lamuelaraventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999;299:152-178.

Rajendran C, Christopher FC, Ramu N, Pazhamalai V, Mohankumar N, Ethiraj S. Studies on Phytochemical screening, antioxidant activity and extraction active Compound (2-Hydroxy 4-Methoxy Benzaldehyde) from rhizome extract of Decalepis hamiltonii Wight and Arn. International Journal of Current Biotechnology. 2014;2:31-39.

Gagandeep Kaur, Pranav Kumar, Uma Ranjan Lal, Ashish Suttee. Phytochemical and biological analysis of Tinospora cordifolia. International Journal of Toxicological and Pharmacological Research. 2016;8(4):297-305.

Kumar Hemant, Goswami Mradul, Yadav Sanjay, Rao CV. Evaluation of in-vitro antioxidant activity in Ficus religiosa (L.) Leaves. International Journal of Research in Pharmacy and Science. 2011;1(2):102-110.

Re R, Pellegrini N, Proteggente A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 1999;26:1231-37.

Oyaizu M. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition. 1986;44:307-315.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitative of antioxidant capacity through the formation of a Phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical biochemistry. 1999;269:337-341.

Nikolic R, Miletic NMR, Neskovic MN. Effects of Cytokinins on in vitro seed germination and early seedling morphogenesis in Lotus corniculatus L. Journal of Plant Growth Regulation. 2006;25:187-194.

Tejavathi DH, Raveesha HR, Shobha K. Organogenesis from the culture of Nothapodytes foetida (Wight) Sleumer raised on TDZ supplemented media. Indian Journal of Biotechnology. 2012;11:205-209.

Azizan MNAB. The effect of BAP and NAA treatment on micropropagation of Cucumis sativus.L. International Journal of Science and Research. 2017;6(11):171-176.

Osman, NI, Sidik NJ, Awal A. Effects of variations in culture media and hormonal treatments upon callus induction potential in endosperm explant of Barringtonia racemosa L. Asian Pacific Journal of Tropical Biomedicine. 2016;6:143-147.

George EF. Plant Propagation by tissue culture. Handbook and Directory of commercial laboratories. Exegeties Ltd. Eversely, Basingstoke, England; 2000.

Ying-Hua Su, Yu-Bo Liu, Xian-Sheng Zhang. Auxin–Cytokinin Interaction Regulates Meristem Development. Molecular Plant. 2011;4(4):616–625.

Kaveri S and Srinath Rao. In vitro seed germination and embryo culture in Nothapodytes foetida (Wight) Sleumer. International Letters of Natural Sciences. 2015;48:23-31.

Bennett IJ, Mc Comb JA, Tonkin CM, Mc. David DAJ. Alternating cytokinins in multiplication media stimulates in vitro shoot growth and rooting of Eucalyptus globules. Labill. Annals of Bot. 1994;74(1):53–58.

Mylona P, Dolan L. The root meristem. In: Mc Manus MT, Weit BE (eds) Meristematic tissues in plant growth and development. Sheffield Academic, Sheffield. 2002;279–292.

Greenwell ZL, Ruter JM. Effect of glutamine and arginine on growth of Hibiscus moscheutos “in vitro”. Scientific Article. 2018;393-400.

Fulzele DP, Satdive RK, Pol BB. Untransformed root cultures of Nothapodytes foetida and Production of Camptothecin. Plant Cell, Tissue and Organ Culture. 2002;69(3):285-288.

Castro AHF, Braga KDQ, Marinez de Sousa F, Coimbra MC, Chaga RC. Callus induction and bioactive phenolic compounds production from Byrsonimaver bascifolia (L.) DC. (Malpighiaceae). Rev. Ciênc. Agron. 2016;47(1):143-151.

Khan Nazeerullah, Kumar Sunil, Singh Rishi Pal, Dhankhar Neelam. Preliminary phytochemical screening, fingerprinting and pharmacognostic evaluation of Nothapodytes nimmoniana leaves, stem and root collected from different geographical region. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2012;3(1):362.

Altemimi A, Lakhssassi NN, Baharlouei A, Watson DG, Lightfoot AD. Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts. Plants. 2017;6(42):1-23.

Dixit A, Gayakwad S, Shirolkar A, Warkad S, Devlae A, Murthy SN, Pawar S. Phytochemical characterization and cells based analysis of bioactive components of Nothapodytes nimmoniana (J. Graham). International Journal of Current Microbiology and Applied Sciences. 2015;Spl Issue-2:18-37.

Uthpala T, Raveesha HR. Studies on antioxidant and antibacterial activity of Cissampelos pareira (L.). International Journal of Advanced Scientific Research and Management. 2019;4(4):35-42.

Liu YY, Liu CJ. Determination of total alkaloids in different parts of Actinidia arguta by Spectrophotography. International Conference on Materials, Environmental and Biological Engineering. 2015;127-131.

Lu Y, Foo YL. Antioxidant and free radical scavenging activities of selected medicinal herbs. Journal of Life Science. 2000;66:725-735.

Rao P, Nataraj HR, Subba Rao V. Madhunapantula. Phytochemical analysis and anti-oxidant activity of Nothopodytes nimmoniana (grah.) Mabb root and leaf. World Journal of Pharmaceutical Research. 2017;6(15):618-628.

Krishnan V, Ahmad S, Mahmood M. Antioxidant potential in different parts and callus of Gynura procumbens and different parts of Gynura bicolor. BioMed Research International. 2015;1-7.

Datkhile, Pratik P Durgawale, Madhavi N. Patil, Rohit D. Vhaval, Tejasvi S. Khamkar. Evaluation of in vitro antioxidant and free radical scavenging properties of different parts of medicinal plant Nothapodytes foetida (Family: Icacinaceaae). International Journal of Scientific Research. 2016;5(5):80-84.

Anugraha S, Vennila L, Asaikumar L, Vijayakumar N, Panbilnathan A, Ajay Krishnan U, Arya R. In-vitro antioxidant activity of methanolic leaf and root extracts of Elephantopus scaber. Journal of Pharmacognosy and Phytochemistry. 2019;8(3):404-412.