MYCOSYNTHESIZE OF AG-NANOPARTICLES BY Penicillium expansum AND ITS ANTIBACTERIAL ACTIVITY AGAINST BACTERIAL PATHOGENS
RAGHDA S. ISMAIL *
Department of Botany and Microbiology, Faculty of Science, Benha University, Qalubiya Governorate, 13511, Egypt
REYAD M. EL-SHARKAWY
Department of Botany and Microbiology, Faculty of Science, Benha University, Qalubiya Governorate, 13511, Egypt
BASMA H. AMIN
The Regional Centre for Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt
MAHMOUD A. SWELIM
Department of Botany and Microbiology, Faculty of Science, Benha University, Qalubiya Governorate, 13511, Egypt
*Author to whom correspondence should be addressed.
Abstract
Multidrug-resistant pathogens have promoted research of nontraditional antibiotic agents. Several classes of nano-antibiotics have affirmed their effectiveness against various eukaryotic microorganisms including bacteria, viruses and fungi. The production of silver nanoparticles using Penicillium expansum was investigated. Ag-nanoparticles were characterized by UV–visible spectrum, energy dispersive X-ray, zeta sizer Nano S90 and transmission electron microscopy. Biosynthesize Ag+ nanoparticles were confirmed by 420 nm corresponding to a surface plasmon resonance. EDX has confirmed the presence of the silver element. TEM micrographs showed irregular, spherical, hexagonal and monodispersed nanoparticles of 9–18 nm. Bio-Ag nanoparticles showed a significant antibacterial effect on Methicillin-Resistant Staphylococcus aureus RCMB 010010. The results showed a cytotoxicity activity on the human breast cancer (MCF-7) cell line in a concentration-dependent manner. Thus, biosynthesized Ag-nanoparticles are eco-friendly antibacterial and antitumor compounds.
Keywords: Silver nanoparticles, antibacterial activity, ultrastructure, cytotoxicity.