MOLECULAR VERIFICATION OF SPECIES IDENTITY OF SOME ISOLATES OF THE GENUS Fusarium DEPOSITED IN THE PHYTOPATHOGEN COLLECTION IN UZBEKISTAN
A. G. SHERIMBETOV *
Institute of Genetics and Plants Experimental Biology, Academy of Sciences of Uzbekistan, Tashkent Region, Kibray District, Yukori-Yuz 111226, Uzbekistan
B. SH. ADILOV
Institute of Genetics and Plants Experimental Biology, Academy of Sciences of Uzbekistan, Tashkent Region, Kibray District, Yukori-Yuz 111226, Uzbekistan
Z. N. KADIROVA
Institute of Genetics and Plants Experimental Biology, Academy of Sciences of Uzbekistan, Tashkent Region, Kibray District, Yukori-Yuz 111226, Uzbekistan
T. X. MAKHMUDOV
Institute of Genetics and Plants Experimental Biology, Academy of Sciences of Uzbekistan, Tashkent Region, Kibray District, Yukori-Yuz 111226, Uzbekistan
A. B. MAMBETNAZAROV
Institute of Genetics and Plants Experimental Biology, Academy of Sciences of Uzbekistan, Tashkent Region, Kibray District, Yukori-Yuz 111226, Uzbekistan
D. R. RUZMETOV
Institute of Genetics and Plants Experimental Biology, Academy of Sciences of Uzbekistan, Tashkent Region, Kibray District, Yukori-Yuz 111226, Uzbekistan
U. X. YULDASHOV
Institute of Genetics and Plants Experimental Biology, Academy of Sciences of Uzbekistan, Tashkent Region, Kibray District, Yukori-Yuz 111226, Uzbekistan
E. Y. KARIMOV
Institute of Genetics and Plants Experimental Biology, Academy of Sciences of Uzbekistan, Tashkent Region, Kibray District, Yukori-Yuz 111226, Uzbekistan
*Author to whom correspondence should be addressed.
Abstract
Traditional fungal taxonomy has been based on morphological characteristics, but identification of the species level of some fungal strains belonging to the genus Fusarium using classical methods is difficult and it has limitations in terms of sensitivity and specificity.
The main purpose of this study was molecular genetic verification of the species identity of some Fusarium isolates deposited in the phytopathogen collection in Uzbekistan.
The β‐tubulin-sequence based verification of species identity of 11 Fusarium isolates deposited in the phytopathogen collection in Uzbekistan confirmed initial morphological species identification of 8 strains (73%), while species identity of remaining 3 strains (27%) has been corrected.
These results establish a molecular framework for further efforts to validate species identity of a fungal isolates in a fungal germplasm culture collection in Uzbekistan and other Central Asia countries.
Keywords: Phytopathogen, genus, Fusarium, DNA, PCR, plant.