IMPROVED COWPEA (Vigna unguiculata L. WALP.) RHIZOBIUM TOLERATE TO DROUGHT AND SALINITY CONDITIONS USING CO-INCULATION

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Published: 2020-11-30

Page: 78-85


KADHIMIYAH JAWAD ABDULLAH Al- MANSOR *

Department of Soil Sciences and Water Resources, College of Agriculture, Basra University, Iraq

ABDAL ZAHRA TAHATHAHER

Department of Soil Sciences and Water Resources, College of Agriculture, Basra University, Iraq

*Author to whom correspondence should be addressed.


Abstract

The study was conducted to find out the effect of adding a mixture inoculation  and its tolerance to salinity and lack of moisture on the growth of cowpea plant and its absorption of  N , P and K nutrients,  After planting and growing  cowpea plant for a period of 60 days, then the plants  were harvested and dried at 70°C for 48 hours, and their dry weight was taken, then milled, sifted and digested  , then N, P and K were measured, and the roots of the plant was separated using a light water current and were count  the number of root nodules for each plant.

The results showed that:

1- The mixed inoculation treatment (I7) increased the rate of dry weight, root nodule number and nitrogen, phosphorus and potassium concentration in cowpea plant.

2- An increase in salinity levels reduced the rate of dry weight, average root nodule number, nitrogen, phosphorus and potassium concentration in cowpea plant and the same results were obtained  with reducing moisture condition.

3- The mixed inoculation were increase the tolerance of cowpea plant to the salinity and drought condition led to an increase in N , P , K absorption  and enhances the root nodules formation and increase dry weight .

Keywords: Rhizobia, cowpea, tolerance, salinity, drought.


How to Cite

MANSOR, K. J. A. A.-., & TAHATHAHER, A. Z. (2020). IMPROVED COWPEA (Vigna unguiculata L. WALP.) RHIZOBIUM TOLERATE TO DROUGHT AND SALINITY CONDITIONS USING CO-INCULATION. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(57-58), 78–85. Retrieved from https://ikprress.org/index.php/PCBMB/article/view/5611

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References

Friel CA, Friesen ML. Legumes modulate allocation to rhizobial nitrogen fixation in response to factorial light and nitrogen manipulation. Front. Plant Sci. 2019;10: 1316. DOI: 10.3389/fpls.2019.01316

Zahran, HH Rhizobium–legume symbiosis and fixation under severe conditions and in an arid climat. Department of Botany, Faculty of Science, Beni-suef, 62511 Egypt. Microbiology and molecular Biology reviews, 1999,1092-2172/99 /$04.0010. American Society for Microbiology. 1999; 63(4):968-989.

Al-Tawaha ARM, Al-Tawaha A, Sirajuddin SN, McNeil D, Othman YA, Al-Rawashdeh IM, Amanullah, Imran, Qaisi AM, Jahan N, Shah MA, Khalid S, Sami R, Rauf A, Thangadurai D , Sangeetha J, S Fahad, Youssef RA, Al-Taisan WA, Al-Taey DKA. Ecology and adaptation of legumes crops. IOP Conf. Series: Earth and Environmental Science. 2020;492:012085.

Al-Sherif EM. Ecological studies on the flora of some aquatic systems in Beni-suef district. M.Sc. Thesis. Cairo university (Beni-Suef Branch), Beni-Suef, Egypt; 1998.

Thilakarathna MS, Chapagain T, Ghimire B, Pudasaini R, Tamang BB, Gurung K, Choi K, Rai L, Magar S, Bishnu BK, Gaire S, Raizada MN. evaluating the effectiveness of rhizobium inoculants and micronutrients as technologies for nepalese common bean smallholder farmers in the real-world context of highly variable hillside environments and indigenous farming practices . Agriculture J. 2019;9: 20.

Al-Taey DKA, Majid ZZ. The activity of antioxidant enzymes and NPK contents as affected by water quality, Kinetin, Bio and organic fertilization lettuce (Lactuca sativa L). The Iraqi Journal of Agricultural Science. 2018;49(3):506-518.

Rahman M, Islam M, Bhuiyan M, Khanam D, Hossain A, Rahman A. Effect of rhizobia inculum with and with out chemical fertilizer on chikpen in haqueps. Bangladesh . J. Agric. Sci. 1994;21(2): 273-277.

Slomy AK, Jasman AK, Kadhim FJ, AL-Taey DKA, Sahib MR. Study Impact of Some Biofactorson The Eggplant Solanum melongena L. vegetative characteristics under glass houses conditions. Int. J. Agricult. Stat. Sci. 2019; 15(1):371-374.

Rydlova J, Puschel D. Arbuscular mycorrhiza, but not hydrogel, alleviates drought stress of ornamental plants in peat-based substrate. Pplied Soil Ecology. 2020; 146:1033942. Available:https://doi.org/10.1016/j.apsoil. 2019.103394

White J, Prell J, James EK, Poole P. Nutrient sharing betweensymbionts. Plant Physiol. 2007;144:604–614.

Parween T, Bhandari P, Jan S, uzzafar M, Fatma T, Raza SK. Role of bioinoculants as plant growth-promoting microbes for sustainable agriculture. Agriculture; 2017. DOI: 10.1007/978-981-10-5589-8_9

Pereira S , Mucha Â, Gonçalves B, Bacelar E, Látr A, H Ferreira, Oliveira I, Rosa E, Marques G. Improvement of some growth and yield parameters of faba bean (Vicia faba) by inoculation with rhizobium laguerreae and arbuscularmycorrhizal fungi. Plant Sciences, Sustainable Farming Systems and Food Quality. 2019;70(7).

Rocha I, Souza‐Alonso P, Pereira G, Ma Y, Vosátka M, Freitas H, Oliveira RS. Using microbial seed coating for improving cowpea productivity under a low‐input agricultural system. J. The Scinceof FoodandAgricaltur. 2019;100(3).

Shalini, Srivastara R. Screening for antifungal activity of pseudomonas fluorescens against phyto pathogenic fungi. Joural of Microbiology. 2008;5(2).

Beck DP, Materon LA, F Afandi. Practical rhizobium legume technology manual technical. ICARDA, Syria. 1993;19.

Date, RA. Microbiological problems in the inoculation and nodulation of legumes. Plant and Soil. 1970;32:703-725.

Date RA. Inoculated legumes in cropping systems of the tropics. Field crops Res. 2000;65:123–136.

FNCA forum for nuclear cooperation in asia. Bio fertilizer manual. Japan Atomic Industrial Forum (JAIF), Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan. FNCA; 2006. Available:http://www.fnca.jp/english/index.html

Minaxi J Saxena S Chandra, Nain L Synergistic effect of phosphate solubilizing rhizobacteria and arbuscularmycorrhiza on growth and yield of wheat plants. Journal of Soil Science and Plant Nutrition. 2013; 13(2):511-525.

Santachiara G, Salvagiottib F, Rotundoa JL. Nutritional and environmental effects on biological nitrogen fixation in soybean: A meta-analysis. Field Crops Research. 2019; 240;106–115.

Vriezen JAC, de Bruijn FJ, Nusslein K. Responses of rhizobia to desiccation in relation to osmotic stress, oxygen, and temperature. Applied and Environmental Microbiology. 2007;73:3451–59.

Korir H, Mungai NW, Thuita M, Hamba Y Masso C. Co-inoculation effect of rhizobia and plant growth promoting rhizobacteria on common bean growth in a low phosphorus soil. Front. Plant Sci. 2017;8: 141. DOI: 10.3389/fpls.2017.00141

Figueiredo B, Burity HA, de França FP. Water deficit stress effects on N2 fixation in cowpea inoculated with different Bradyrhizobium strains. Can. J. Plant Sci.1998 Available:www.nrcresearchpress.com by 185.180.63.2 on 12/29/19

Kasper S, Christoffersen B, Soti P, Racelis A. Abiotic and biotic limitations to nodulation by leguminous cover crops in south texas. Agriculture J. 2019;9:209.

Hack CM, Porta M, Schäufele R, Grimoldi AA. Arbuscular mycorrhiza mediated effects on growth, mineral nutrition and biological nitrogen fixation of Melilotusalba Med. in a subtropical grassland soil. Applied Soil Ecology. 2019; 134;38-44. DOI: org/10.1002/jsfa.10117

Lopes EAP, Silva A, Mergulhao A, Silva E, Santiago A, Figueiredo M. Co-Inoculation of growth promoting bacteria and glomusclarum in micropropagated cassava plants. Rev. Caatinga Mossoró. 2019;32(1): 152–166.