EFFECT OF AUXINS (IAA, IBA AND NAA) ON CLONAL PROPAGATION OF Solanum procumbens STEM CUTTINGS
PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY,
Volume 21, Issue 55-56,
Solanum procumbens Lour. is a precious medicinal plant with plenty applications in medicine and pharmacy. Auxin is a plant hormone which is critical to the induction and growth of roots of cuttings. In this study, an experiment was conducted to evaluate the effects of indole acetic acid (IAA), indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA) on the rooting and sprouting of S. procumbens stem cuttings. The experiment involved four distinctive concentrations of IAA (500, 1000, 1500 and 2000 ppm), IBA (500, 1000, 1500 and 2000 ppm) and IBA + NAA (250 + 250, 500 + 500, 750 + 750 and 1000 + 1000 ppm) along with the control group used to treat S. procumbens cuttings. The experiment applied the randomized complete block (RCB) method with 3 replications. The results showed that IAA, IBA and NAA had a remarkable influence on rooting and sprouting abilities of S. procumbens cuttings. The best records of sprouting rate (92.34%), root number (32.25), root length (6.8 cm), root weight (0.574 g), sprout length (11.7 cm) and leaf-pair number (7.5) were obtained in cuttings treated with IBA at 500 ppm. Furthermore, the beneficial effects of auxins wore off as their concentrations increased. In general, this study emphasizes that IBA at 500 ppm is the most effective auxin in terms of stimulating rooting and sprouting in S. procumbens cuttings. The results of the study also promise to create a supply of high-quality S. procumbens seedlings for gardeners.
- Solanum procumbens
- stem cuttings
How to Cite
Hai NX, Nhan NT, Mai NTT. Chemical constituents isolated from the whole plant of Solanum procumbens. Science and Technology Development Journal-Natural Sciences. 2018;2(6):134-138.
Hien TTT, Tuan HA, Huong DP, Luong HV, Mai NTT, Tai BH, Kiem PV. Two new steroidal saponins from Solanum procumbens. Natural Product Communications. 2018;13(10):1271-1274.
Tran TTT, Dang HP,Nguyen TN. Chemical constituents from methanolic extract of Solanum procumbens Lour (Solanaceae). Vietnam Journal of Science, Technology and Engineering. 2019;61(3):9-11.
Thai NP, Trung LV, Hai NK,Huynh L. Protective efficacy of Solanum hainanense Hance during hepatotoxicity in male mice with prolonged and small oral doses of trinitrotoluene. Journal of Occupational Health. 1998;40(4):276-278.
Chi VV. Dictionary of Vietnamese medicinal plants, Medical Publishing House, Hanoi. [In Vietnamese]. 2012; 1:1675.
Nadeem M, Palni LMS, Purohit AN, Pandey H, Nandi SK. Propagation and conservation of Podophyllum hexandrum Royle: An important medicinal herb. Biol. Conserv. 2000;92(1):121-129.
Saumya MT, Surendra T, Hrideek TK. Vegetative propagation for different physiological ages of Embelia ribes cuttings in different seasons. Res. J. Agric. For. Sci. 2014;2(2):8-12.
Raju NL, Prasad MNV. Influence of growth hormones on adventitious root formation in semi-hardwood cuttings of Celastrus paniculatus Wild.: A contribution for rapid multiplication and conservation management. Agrofor. Syst. 2010;79:249-252.
Blythe EK, Sibley JL, Tilt KM, Ruter JM. Methods of auxin application in cutting propagation: A review of 70 years of scientific discovery and commercial practice. Journal of Environmental Horticulture. 2007;25(3):166-185.
Ludwig-Müller J. Indole-3-butyric acid in plant growth and development. Plant Growth Regulation. 2000;32(2-3):219-230.
Shahzad U, Kareem A, Altaf K, Zaman S, Ditta A, Yousafi Q, Calica P. Effects of auxin and media additives on the clonal propagation of guava cuttings (Psidium guajava L.) Var. Chinese Gola. J. Agri. Sci. Food Res. 2019;10(3):265.
Sevik H, Guney K. Effects of IAA, IBA, NAA, and GA3 on rooting and morphological features of Melissa officinalis L. stem cuttings. The Scientific World Journal; 2013.
Yusnita Y, Jamaludin J, Agustiansyah A, Hapsoro D. A combination of IBA and NAA resulted in better rooting and shoot sprouting than single auxin on malay apple [Syzygium malaccense (L.) Merr. & Perry] stem cuttings. AGRIVITA, Journal of Agricultural Science. 2017;40(1):80-90.
Topacoglu O, Sevik H, Guney K, Unal C, Akkuzu E, Sivacioglu A. Effect of rooting hormones on the rooting capability of Ficus benjamina L. cuttings. Šumarski list. 2016; 140(1-2):39-44.
Kontoh IH. Effect of growth regulators and soil media on the propagation of Voacanga africana stem cuttings. Agroforestry Systems. 2016;90(3):479-488.
Kesari V, Krishnamachari A, Rangan L. Effect of auxins on adventitious rooting from stem cuttings of candidate plus tree Pongamia pinnata (L.), a potential biodiesel plant. Trees. 2009;23(3):597-604.
Husen A, Iqbal M, Siddiqui SN, Sohrab SS, Masresha G. Effect of indole-3-butyric acid on clonal propagation of mulberry (Morus alba L.) stem cuttings: Rooting and associated biochemical changes. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 2017; 87(1):161-166.
Wahab F, Nabi G, Ali N, Shah M. Rooting response of semi-hardwood cuttings of guava (Psidium guajava L.) to various concentrations of different auxins. Journal of Biological Sciences. 2001;1(4):184-187.
Waheed A, Hamid FS, Ahmad H, Abbassi FM, Aslam S, Shah AH, Ahmad N, Naheed Z, Ali H, Khan N. Effect of indole butyric acid (IBA) on early root formation (tomato ‘Sahil’hybrid) cuttings. J. Mater. Environ. Sci. 2015;6(1):272-279.
Aghdaei M, Nemati SH, Samiee L, Sharifi A. Effect of rooting medium, cutting type and auxin on rooting of pepino (Solanum muricatum Aiton) cutting. Applied Ecology and Environmental Research. 2019;17(5): 10357-10369.
Kamila PK, Das PK, Mohapatra PK,Panda PC. Effect of auxins on rooting of stem cuttings in Hypericum gaitii. Journal of Herbs, Spices & Medicinal Plants. 2020; 26(4):423-434.
Doungous O, Minyaka E, Medza-Mve SD, Medueghue AF, Ngone MA, Simo C, Nsimi AM. Improving propagation methods of Gnetum africanum and G. buchholzianum from cuttings for rapid multiplication, domestication and conservation. Agroforestry Systems. 2019; 93(4):1557-1565.
Yan SP, Yang RH, Wang F, Sun LN, Song XS. Effect of auxins and associated metabolic changes on cuttings of hybrid aspen. Forests. 2017; 8(4):117.
Yan YH, Li JL, Zhang XQ, Yang WY, Wan Y,Ma YM, Zhu YQ, Peng Y, Huang LK. Effect of naphthalene acetic acid on adventitious root development and associated physiological changes in stem cutting of Hemarthria compressa. PLoS One. 2014; 9(3):90700.
Tworkoski T, Takeda F. Rooting response of shoot cuttings from three peach growth habits. Scientia Horticulturae. 2007;115(1): 98-100.
Hartmann HT, Kester DE. Plant propagation: Principles and practices. Prentice-Hall; 1975.
Henrique A, Campinhos EN, Ono EO, Pinho SZD. Effect of plant growth regulators in the rooting of Pinus cuttings. Brazilian Archives of Biology and Technology. 2006;49(2):189-196.
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