ALKALOIDS PROFILING AND ACETYLCHOLINESTERASE INHIBITORY ACTIVITY OF Corydalis SPECIES
IVAN G. IVANOV *
Department of Organic Chemistry and Inorganic Chemistry, University of Food Technologies, Plovdiv, 26 Maritza Blvd., 4002, Plovdiv, Bulgaria
RADKA Z. VRANCHEVA
Department of Analytical Chemistry and Physical Chemistry, University of Food Technologies, Plovdiv, 26 Maritza Blvd., 4002, Plovdiv, Bulgaria
INA Y. ANEVA
Institute of Biodiversity and Ecosystem Research at the Bulgarian Academy of Science, 1113 Sofia, Bulgaria
IVAYLA N. DINCHEVA
AgroBioInstitute, Agricultural Academy, 8 Dr. Tsankov Blvd, 1000, Sofia, Bulgaria
ILIAN K. BADJAKOV
AgroBioInstitute, Agricultural Academy, 8 Dr. Tsankov Blvd, 1000, Sofia, Bulgaria
ATANAS I. PAVLOV
Department of Analytical Chemistry and Physical Chemistry, University of Food Technologies, Plovdiv, 26 Maritza Blvd., 4002, Plovdiv, Bulgaria. and Department of Industrial Microbiology, Laboratory of Applied Biotechnologies, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd., 4000 Plovdiv, Bulgaria .
*Author to whom correspondence should be addressed.
Abstract
The aim of this study was GC-MS analysis of alkaloid profiles of different aerial parts (leaves, stems and flowers) of three Corydalis species, naturally grown in Bulgaria (Corydalis solida (L.) Clairv., Corydalis marschalliana (Willd.) Pers. and Corydalis bulbosa (L.) DC.), as well as analysis of the acetylcholinesterase inhibitory activity of obtained alkaloid extracts. Thirteen isoquinoline alkaloids were identified, as the principal ones were: protopines (protopine, allocryptopine and corycavidine); aporphines (corydine, bulbocapnine and nantenine); benzylisoquinolines (N-methyllaurotetanine); protoberberines (stylopine and canadine); spirobenzylisoquinolines (parfumine, fumarophycine and bicuculline); and phenanthrenes (thalicthuberine). Investigated alkaloid extracts showed significant acetylcholinesterase (AChE) inhibitory activity in comparison to galanthamine (IC50 0.31 mg/mL), as the highest activity possessed leave extracts of Corydalis solida and C. marschalliana (IC50 0.36 mg extract /mL).
Keywords: Isoquinoline alkaloids, Corydalis, acetylcholinesterase inhibitory activity.