INFLUENCE OF THE SUPRAMOLECULAR COMPLEX OF GLYCYRRHIZIC ACID WITH QUERCETIN ON AGE-RELATED FUNCTIONAL CHANGES IN RAT BRAIN MITOCHONDRIA
SURAYYO DALIMOVA
Department of Biochemistry, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
SHERALI KUZIEV
Department of Biochemistry, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
GULBAKHOR UMAROVA
Department of Biochemistry, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
GUZAL MUKHAMMADJONOVA
Department of Biochemistry, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
MUSLIMA YUNUSOVA
Department of Biochemistry, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
NIGORA KHAMDAMOVA
Department of Biochemistry, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
FARKHOD ESHBOEV *
Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, M. Ulugbek St. 77, 100170, Tashkent, Uzbekistan
*Author to whom correspondence should be addressed.
Abstract
Background: Over the last decades, a large amount of literature regarding lipid peroxidation has shown its important role in cell biology and human health. Previous studies have shown that plant polyphenols impact positively to the cell mitochondria. Consequently, an investigation of the effect of the supramolecular complex of the natural flavonoid quercetin with glycyrrhizic acid (GA+Qu) may well provide useful leads in the discovery of new pharmaceuticals.
Methods: Mitochondria were isolated from the brain of rats in which 3, 6, 9, 12, and 15 months of age and the intensity of lipid peroxidation, respiration, and oxidative phosphorylation, the activity of enzymes of the respiratory chain, and protein biosynthesis were investigated.
Results: Lipid peroxidation (LPO) and changes of the functional state (respiration, oxidative phosphorylation, the activity of respiratory chain enzymes, protein biosynthesis) of brain mitochondria of rats in different ages and the effect of the supramolecular complex of the natural flavonoid quercetin (Qu) with glycyrrhizic acid (GA) on these processes were studied in this work. It was revealed that the content of malonedialdehyde (MDA) in mitochondria of 3,6, 9,12, and 15-month-old rats increase which right correlates together a growth of the age of the animal. It was found that not only the intensity of LPO changes with the age of the animal, also changes the kinetics of the process. A decrease in respiration, oxidative phosphorylation, activity of respiratory enzymes, and protein biosynthesis was observed in the mitochondria of the brain of 12 and 15-month old rats. The introduction of the supramolecular complex of the glycyrrhizic acid with the flavonoid quercetin decreased the content of the MDA in 12 and 15-month-old animals while restoring the studied processes in mitochondria.
Conclusion: According to the results of the study, the supramolecular complex of Ga + Qu may be used to create a geroprotective drug.
Keywords: Lipid peroxidation, malonedialdehyde, quercetin, glycyrrhizic acid, mitochondria.