CARBOHYDRATE COMPOSITION OF OATS FRUIT (Avena sativa L.)
NURULLAEVA DILOBAR HAMID KIZI *
Department of Pharmacognosy, Tashkent Pharmaceutical Institute, Uzbekistan
FARMANOVA NODIRA TAKHIROVNA
Department of Pharmacognosy, Tashkent Pharmaceutical Institute, Uzbekistan
POVYDYSH MARIA NIKOLAEVNA
Department of Pharmacognosy, St. Petersburg State Chemical Pharmaceutical University, Russia
ORLOVA ANASTASYA ANDREEVNA
Department of Pharmacognosy, St. Petersburg State Chemical Pharmaceutical University, Russia
NURULLAEVA BARNO RAVSHANKIZI
Department of Medical Radiology, Tashkent Pediatric Medical Institute, Uzbekistan
*Author to whom correspondence should be addressed.
Abstract
This work is devoted to the study of the carbohydrate complex of oats. As a result of the study, the presence of alcohol-soluble sugars, water-soluble polysaccharides, pectin substances and hemicelluloses was established. The object of the study was crushed fruits of sown oats. The alcohol-soluble sugars were extracted with 82°С boiling alcohol. Extraction of the sum of water-soluble polysaccharides from the raw material was carried out with water. A 96% ethanol precipitation reaction was used to detect polysaccharides. The sum of the water-soluble polysaccharides was quantified by the gravimetric method. Separate fractions of polysaccharides were isolated from the defatted cake. Pectin substances (PV) were recovered with a mixture of 0.5% oxalic acid and ammonium oxalate (1:1) and hemicellulose with 10% sodium hydroxide solution. IR spectra of isolated polysaccharides were also studied.
Keywords: Alcohol-soluble sugars, Avena sativa, pectin, polysaccharides, starch.