PRODUCTION OF LIGNOCELLULOLYTIC ENZYMES BY Aspergillus niger FROM BROWN MID RIB Sorghum VARIETIES
PALAKEERTI SRINIVAS KUMAR
Centre for Bio-Technology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Kukatpally, Telangana, India
VANI GANDHAM
Centre for Bio-Technology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Kukatpally, Telangana, India
ADDEPALLY UMA *
Centre for Bio-Technology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Kukatpally, Telangana, India
A. V. UMAKANTH
ICAR-Indian Institute of Millets Research, Rajendra Nagar, Hyderabad, Telangana, India
*Author to whom correspondence should be addressed.
Abstract
The present study reported with the production of enzyme cellulases (CMCase, Fpase and β-glucosidase) and xylanase by submerged fermentation using Aspergillus niger and sorghum hybrid non-BMR CSH 22 variety & low lignin BMR 22 and 24 substrates were evaluated after pre-treating with H2SO4 & NaOH. The highest cellulose and low lignin reported pretreatment conditions were applied for substrates and used the same in the fermentation. Maximum CMCase, FPase, and β-glucosidase production was observed for pretreated BMR 22 Sorghum biomass with values 30.5 ± 3.75 IU/ml, 2.74±0.34 IU/ml, and 9.6 ± 0.37 IU/ml respectively. But maximum xylanase production 15.8±1.75 IU/ml was observed while using untreated CSH 22 sorghum biomass as substrate. Results indicate low lignin mutant BMR22 and 24 substrates were promising substrates for the production of cellulase enzymes.
Keywords: Sorghum, Brown Mid Rib, cellulose, xylanase, submerged fermentation