ANTIOXIDANT AND ANTIINFLAMMATORY ACTIVITY OF CHITOSAN ENCAPSULATED OMEGA 3-6-9
S. VIDYASHRI
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
A. LAKSMINARAYANAN *
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
S. RAJESHKUMAR
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
T. LAKSHMI
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Omega 3-6-9 fatty acid supplements contain omega 3, omega 6 and omega 9 fatty acids in the preferred proportions, usually 2:1:1. It is important to maintain a low omega 6 and 3 ratio to reduce inflammation. There must be a balance to be maintained between the requirements and intake of omega 3, 6 and 9. A balanced proportion is necessary for maintaining a healthy composure and preventing chronic diseases. It is preferred to maintain the ratio below 1:4. Chitosan is mainly used as a drug delivery system by encapsulating the substance to be delivered. Since chitosan is polycationic, It interacts with the mucous membrane which is negatively charged and adheres to it and thus increases the time of contact for the drug to penetrate through it. The omega 3-6-9 molecules are transported into the cell using chitosan encapsulation.
Aim: To evaluate the anti-inflammatory and antioxidant activity of chitosan encapsulated omega 3-6-9.
Methodology: Preparation of chitosan encapsulated omega 3-6-9 is done using a magnetic stirrer. The chitosan and omega 3-6-9 oil are mixed in the proportion of 5:0.5 mg followed by the testing of its antiinflammatory and antioxidant activities by protein denaturation and DPPH assay respectively.
Results: Chitosan encapsulated omega 3-6-9 exhibit significant anti-inflammatory and antioxidant activity. Both the antioxidant and antiinflammatory activity of the solution was seen to have a gradual increase with increased concentration. Maximum activity is seen at 50 μL.
Conclusion: From the present study it can be concluded that chitosan encapsulated omega 3-6-9 have a good anti-inflammatory and antioxidant activity at high concentrations. This is a relatively novel concept but has good potential in the pharmaceutical industry.
Keywords: Omega 3-6-9, chitosan, encapsulation, anti-inflammatory, antioxidant