In vitro EXPLORATION OF THE SIGNIFICANCE OF SALINITY STRESS IN ENHANCING ANTIOXIDANT CAPACITY OF Rauwolfia serpentina (L.) BENTH. ex KURZ (ANGIOSPERMS; APOCYNACEAE)

Main Article Content

PUNEET S. SINGH
MALINI BHATTACHARYYA
BABITA PATNI

Abstract

Rauwolfia serpentina is an endangered plant. The plant tissue culture comes to rescue when the plant has low germination rate and high medicinal value as reported in Rauwolfia. This technique is used not only for mass production of drug at cost affordable levels but also for the conservation of important species. Rauwolfia has great medicinal value due to various indole alkaloids and moreover Resperine is the main constituent. Callus culture was induced from leaf, shoot explants on Murashige and Skoog medium augmented by diverse concentrations of growth regulators for swift commencement of callus and biomass production. Rauwolfia releases many different phenolic compounds and alkaloids which are responsible for the antioxidant activity. Phenolic accumulation is stress marker in plants in response to abiotic stress such as salinity. In this review an attempt is made to highlight the phenylpropanoid pathway and phenolic acid response in Rauwolfia under salinity stress in lab conditions and thus contribute to antioxidant property of the plant. Abiotic stress leads to activation of PAL enzyme which is crucial step in phenylpropanoid pathway and supports synthesis of phenolics which have antioxidant potential of great value. The emphasis of present review is micropropogation of Rauwolfia due to its high therapuetic value and influence of salinity stress on phenylpropanoid pathway. Box plot analyses are performed on PAL enzyme activity in salt stress in callus of Rauwolfia serpentina. These results graphically depicted the PAL enzyme activity data distribution. Additional research of this species should be focussed on in vitro investigations of its pharmaceutical active mechanisms which help in formulation of curative products of high value. Moreover, molecular understanding of salinity stress which influence PAL enzyme and synthesis of phenolics will enhance the large-scale production of phytochemicals for various scientific purposes.

Keywords:
Micropropogation, Rauwolfia, phenylalanine ammonia lyase (PAL), phenolics, phenylpropanoid pathway, salinity

Article Details

How to Cite
SINGH, P. S., BHATTACHARYYA, M., & PATNI, B. (2020). In vitro EXPLORATION OF THE SIGNIFICANCE OF SALINITY STRESS IN ENHANCING ANTIOXIDANT CAPACITY OF Rauwolfia serpentina (L.) BENTH. ex KURZ (ANGIOSPERMS; APOCYNACEAE). PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(15-16), 89-97. Retrieved from https://www.ikprress.org/index.php/PCBMB/article/view/5158
Section
Review Article

References

Deshmukh SR, Ashrit DS, Patil BA. Extraction and evaluation of indole alkaloids from Rauwolfia serpentina for their antimicrobial and antiproliferative activities. Int J Pharm Pharm Sci. 2012;4:329–334.

Poonam AS, Mishra S. Physiological, biochemical and modern biotechnological approach to improvement of Rauwolfia serpentina. J Pharm Biol Sci. 2013;6:73–78.

Siddiqui S, Siddiqui RH. Studies in the alkaloidal constituents of roots of Rauwolfia serpentine. J. Ind. Chem. Soc. 1931;9:539.

Bhausaheb A, Deshmukh, Sarika R, Dhanashree S. Extraction and evaluation of indole alkaloids from Rauwolfia serpentina for their antimicrobial and antiproliferative properties. International Journal of Pharmacy & Pharmaceutical Sciences. 2012;4:329.

Panjikar S, Koepke J. The structure of Rauwolfia serpentina Strictosidine synthase is a novel six-bladed β-propeller fold in plant proteins. The Plant Cell. 2006;8(4): 907-920.

Edeoga HO, Okwu DE, Mbaebre BO. Phytochemical constituent of some Nigerian medicinal plants. Afr. J. Biotechnol. 2005;7:685-688.

Pathania S, Randhawa V, Bagler G. Prospecting for novel plant-derived molecules of Rauwolfia serpentina as inhibitors of Aldose Reductase, a potent drug target for diabetes and its complications. PLoS ONE. 2013;8(4): e61327.

Qureshi SA, Nawaz A, Udani SK, Azmi B. Hypoglycaemic and hypolipidemic activities of Rauwolfia serpentina in alloxan-induced diabetic rats. Int. J. Pharmacol. 2009;5:323-6.

Mitra GC. Studies on the formation of viable and non-viable seeds in Rauwolfia serpentina Benth. Indian J. Exp. Biol. 1976;14(1):5456.

Mitra GC, Kaul KN. In vitro culture of root and stem callus of Rauwolfia serpentina for reserpine. Indian J. Exp Biol. 1970;2:49-51.

Morpurgo R, Rodriguez D. In vitro differential response of the Rauwolfia under sodium chloride stress conditions. Riv. Di Agric. Subtrop. 1987;81:73-77.

Cheng KC, Cheng L. Callus cultures of the three well known Chinese herbs and their medicinal contents. In Proc. of the Baijing (Peking) Symp. Pitman Advances. 1981;4:469.

Anitha S, Ranjitha BD. Reserpine accumulation in NaCl treated Calli of Rauwolfia tetraphylla L. Science Asia. 2006;32:417-419.

Shariff NS, Sudharshana MS, Umesha S, Hari Prasad P. Antimicrobial activity of Rauwolfia tetraphylla and Physalis minimal leaf and callus extracts. Afr. J. Biotechnol. 2006;5:946‒950.

Ganga B, Umamaheswar P, Sambasiva E, Praneeth VS. Evaluation of phytochemical constituents, quantification of total phenolic content, alkaloid content and in-vitro anti-oxidant activity of Rauwolfia root bark. Planta Activa. 2011;11:35.

Salma U, Rahman MSM, Islam S, Haque N, Jubair TA, Haque AKMF. The influence of different hormone concentration and combination on callus induction and regeneration of Rauvolfia serpentina (L.) Benth. Pak J Biol Sci. 2008;11:1638-41.

Nurchgani N, Solichatun S, Anggarwulan E. The reserpine production and callus growth of Indian snake root (Rauvolfia serpentina (L.) Benth. ex Kurz.) cultured by addition of Cu2+. Biodiversitas. 2008;9:177-9.

Maeda H, Dudareva N. The shikimate pathway and aromatic amino acid biosynthesis in plants. Annu Rev Plant Biol. 2012;63:73–105.

Hahlbrock K, Scheel D. Physiology and molecular biology of phenylpropanoid metabolism. Ann. Rev. Plant Physiol, Plant Mol. Biol. 1989;4:347–369.

Herrmann KM, Weaver LM. The shikimate pathway. Annu. Rev. Plant Physiol. Plant Mol. Biol. 1999;50:473–503.

Tzin V, Galili G. The biosynthetic pathways for shikimate and aromatic amino acids in Arabidopsis thaliana. The Arabidopsis Book. 2010;8:e0132.

Gershenzon J, D'Auria, Pichersky E. Biochemistry of plant volatiles. Plant Physiol. 2005;135:1893–1902.

Vogt T. Phenylpropanoid biosynthesis. Mol. Plant. 2010;3:2–20.

Parida A, Das AB, Sanada Y, Mohanty P. Effects of salinity on biochemical components of the mangrove Aegiceras corniculatum. Aqua. Bot. 2004;80:77–87.

Ksouri R, Megdiche W, Debez A, Falleh H, Grignon C, Abdelly C. Salinity effects on polyphenol content and antioxidant activities in leaves of the halophyte Cakile maritima. Plant Physiol. Biochem. 2007;45:244–249.

Hichem H, Mounir D, Naceur A. Differential responses of two maize varieties to salt stress: Changes on polyphenols composition of foliage and oxidative damages. Indust Crops Prod. 2009;30:144–151.

Koukol J, Conn EE. The metabolism of aromatic compounds in higher plants. IV. Purification and properties of the phenylalanine deaminase of Hordeum vulgare. J. Biol.Chem. 1961;236:2692–2698.

Wang W, Vincour B, Altman A. Plant responses to drought, salinity and extreme temperatures towards genetic engineering for stress tolerance. Planta. 2007;218:1-14.

Taïbi K, Taïbi F, Ait, Abderrahim L, Ennajah A, Belkhodja M, Mulet JM. Effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidant defence systems in Phaseolus vulgaris L. S. Afr. J. Bot. 2016;123:34.

Acosta-Motos JR, Ortuño MF, Bernal-Vicente A, Diaz-Vivancos P, Sanchez-Blanco MJ, Hernandez JA. Plant responses to salt stress: Adaptive mechanisms. Agronomy. 2017;7:18.

Chen S, Wu F, Li Y, Qian Y, Pan X, Li F, Wang Y, Wu Z, Fu C, Lin H. NtMYB4 and NtCHS1 are critical factors in the regulation of flavonoid biosynthesis and are involved in salinity responsiveness. Front. Plant Sci. 2019;10:178.

Bistgani ZE, Hashemi M, DaCosta M, Craker L, Maggi F, Morshedloo MR. Effect of salinity stress on the physiological characteristics, phenolic compounds and antioxidant activity of Thymus vulgaris L. and Thymus daenensis Celak. Ind. Crops Prod. 2019;135:311–320.

Rossi L, Borghi M, Francini A, Lin X, Xie DY, Sebastiani L. Salt stress induces differential regulation of the phenylpropanoid pathway in Olea europaea cultivars Frantoio (salt-tolerant) and Leccino (salt-sensitive). J. Plant Physiol. 2016;204:8–15.

Al-Ghamdi AA, Elansary HO. Synergetic effects of 5-aminolevulinic acid and Ascophyllum nodosum seaweed extracts on Asparagus phenolics and stress related genes under saline irrigation. Plant Physiol Biochem. 2018;129:273–284.

Miladinova K, Ivanova K, Georgieva T, Geneva M, Markovska Y. Influence of salt stress on ex vitro growth and antioxidative response of two Paulownia clones. Acad. Georgi Bonchev Str. 2008;21:113.

MacDonald MJ, Cunha GB. A modern view of phenylalanine ammonia-lyase. Biochem. Cell Biol. 2007;85:273–282.

Valifard M, Mohsenzadeh S, Niazi A, Moghadam A. Phenylalanine ammonia lyase isolation and functional analysis of phenylpropanoid pathway under salinity stress in ‘Salvia’ species. Aust. J. Crop Sci. 2015;9:656–665.

Ben-Abdallah SW, Amyot L, Renaud J, Hannoufa A, Lachâal M, Karray-Bouraoui N. Potential production of polyphenols, carotenoids and glycoalkaloids in Solanum villosum Mill. under salt stress. Biologia. 2019;74:309–324.

Perin EC, Da Silva, Messias R, Borowski JM, Crizel RL, Schott IB, Carvalho IR, Rombaldi CV, Galli V. ABA-dependent salt and drought stress improve strawberry fruit quality. Food Chem. 2019;271:516–526.

Perin EC, Da Silva, Messias R, Borowski JM, Crizel RL, Schott IB, Carvalho IR, Rombaldi CV, Galli V. ABA-dependent salt and drought stress improve strawberry fruit quality. Food Chem. 2019;271:516–526.

Navarro JM, Flores P, Garrido C, Martinez V. Changes in the contents of antioxidant compounds in pepper fruits at ripening stages, as affected by salinity. Food Chem. 2006;96:66-73.

Mutlu F, Bozcuk S. Salinity-induced changes of free and bound polyamine levels in sunflower (Helianthus annuus L.) roots differing in salt tolerance. Pak J Bot. 2007;39:1097-10