PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

The present research was carried out to develop an alternative in vitro low-cost culture medium for regeneration of an inimitable medicinal plant Gynura procumbens. The nodal segment has been utilized as explant and six separate types of low-cost medium (LCM) which includes LCM-1 (100 mL coconut water); LCM-2 (150 mL coconut water); LCM-3 (200 mL coconut water); LCM-4 (100 mL banana pulp); LCM-5 (150 mL banana pulp); LCM-6, (200 mL banana pulp) and the complete strength MS medium was employed as a control treatment for the standardization of in vitro low-cost regeneration protocol for the Gynura procumbens. All the culture media were supplemented with 2.0 mg / L BA + 0.5 mg / L NAA for shooting and rooting with 0.5 mg / L IBA. Compared to other low-cost culture media, culture media LCM-2 showed the best results in shoot initiation, shoot multiplication, and root formation. As regards cost analysis, LCM-2 appears to minimize cost (78.22%) of the medium per plantlet compared to the regular standard MS medium. Medium of acclimatization consisting of garden soil + Vermicompost + coconut dust (1:1:1, v / v) demonstrated the best hardening medium with maximum survival rate and robust growth performance. Our findings suggest that the LCM-2 culture medium for the regeneration of Gynura procumbens aseptically is an economically cost-efficient protocol and an alternative to the regular standard MS medium.