EFFECT OF PRE AND POST CULTURE TEMPERATURE TREATMENTS ON DIRECT MICROSPORE EMBRYOGENESIS IN INDIAN CHILLI PEPPER (Capsicum annuum L.)
H. M. ARJUN *
Department of Biotechnology, Nuziveedu Seeds Limited, Survey No 69, Kandlakoya, Medchal, Hyderabad, Telangana, India .
BANDARI RAJESH
Department of Biotechnology, Nuziveedu Seeds Limited, Survey No 69, Kandlakoya, Medchal, Hyderabad, Telangana, India.
SRIPATHI VIJAY GOUD
Department of Biotechnology, Nuziveedu Seeds Limited, Survey No 69, Kandlakoya, Medchal, Hyderabad, Telangana, India.
S. LOKESHA
Department of Biotechnology, Nuziveedu Seeds Limited, Survey No 69, Kandlakoya, Medchal, Hyderabad, Telangana, India .
P. SATEESH KUMAR
Department of Biotechnology, Nuziveedu Seeds Limited, Survey No 69, Kandlakoya, Medchal, Hyderabad, Telangana, India .
*Author to whom correspondence should be addressed.
Abstract
The present study was conducted to determine the effect of the pre and post temperature treatment on embryo induction and green plant regeneration from chilli anther culture and how it is influenced by low temperature treatments. Microspore embryogenesis is an efficient method to produce double haploids in Indian Chilli pepper. The effect of androgenesis for 10 F1 genotypes was evaluated in two different temperature treatments. All the genotypes showed differential expression for two pre cold treatments (fresh, 1 Day cold treatment and 2 Days cold treatment) and post culture cold treatment at 9°C for 8 days. Highest frequency of direct embryogenesis for different genotypes varied between 37.5% to 4.13% in PH53 and PH17 respectively, at 2 days pre cold treatment and 9°C post culture cold treatment. Both pre and post temperature treatment played an important role in direct embryogenesis. Highest rate of embryo induction rate was observed in 2 days cold treatment and 9°C post culture treatment across the genotypes. This clearly indicates pre and post cold temperature treatments has direct influence on frequency of androgenesis.
Keywords: Embryo, anther culture, haploid, induction, direct microspore embryogenesis.