EVALUATION OF THE MYCORRHIZAL STATUS OF ACACIAS IN THE RHAMNA-SIDI BOUATHMAN AND THE HAHA REGIONS IN MOROCCO
S. MAAZOUZI
Laboratoire de Botanique, Biotechnologie et Protection de Plantes, Faculté des Sciences, Université Ibn Tofail, Kénitra, Morocco.
J. AOUJDAD
Centre de Recherche Forestière, HCEFLCD, MAPMDREF, Rabat, Morocco.
K. SELMAOUI
Laboratoire de Botanique, Biotechnologie et Protection de Plantes, Faculté des Sciences, Université Ibn Tofail, Kénitra, Morocco.
S. El GABARDI
Laboratoire de Botanique, Biotechnologie et Protection de Plantes, Faculté des Sciences, Université Ibn Tofail, Kénitra, Morocco.
M. ARTIB
Laboratoire de Botanique, Biotechnologie et Protection de Plantes, Faculté des Sciences, Université Ibn Tofail, Kénitra, Morocco.
S. ELANTRY
Centre de Recherche Forestière, HCEFLCD, MAPMDREF, Rabat, Morocco.
M. OUAJDI
Centre de Recherche Forestière, HCEFLCD, MAPMDREF, Rabat, Morocco.
M. BELLAKA
Centre de Recherche Forestière, HCEFLCD, MAPMDREF, Rabat, Morocco.
B. KERDOUH
Centre de Recherche Forestière, HCEFLCD, MAPMDREF, Rabat, Morocco.
A. OUAZZANI TOUHAMI
Laboratoire de Botanique, Biotechnologie et Protection de Plantes, Faculté des Sciences, Université Ibn Tofail, Kénitra, Morocco.
R. BENKIRANE
Laboratoire de Botanique, Biotechnologie et Protection de Plantes, Faculté des Sciences, Université Ibn Tofail, Kénitra, Morocco.
A. DOUIRA *
Laboratoire de Botanique, Biotechnologie et Protection de Plantes, Faculté des Sciences, Université Ibn Tofail, Kénitra, Morocco.
*Author to whom correspondence should be addressed.
Abstract
The present study was initiated with the aim of determining the diversity of endomycorrhizal fungi leads to the regeneration of forest systems (reforestation of local species). To achieve this, root and soil samplings were carried out under Acacia feet developing in the regions of Rhamna-Sidi Bouathman (Bounaga, Jbilat) and Haha (Amhach,). Spores were isolated from soil samples by wet sieving and the roots were stained with cresyl blue. The observation of the roots shows an arbuscular mycorrhization of all the species of the studied genus Acacia. The frequency of mycorrhization of roots varies from 50% to 83.33%. The highest mycorrhizal intensity was observed in Acacia raddiana roots and the lowest in Acacia aneura roots. Vesicles were not observed and arbuscular contents ranged from 8.85% in Acacia raddiana roots to 0.23% in Acacia aneura roots. The number of AMC spores isolated from different soils varies from 13 to 29 spores per 100g of soil in the bare soil of the sites surveyed and from 13 to 32 spores per 100 g of soil in the rhizosphere of the acacias. These spores represent 41 species of MA fungi that belong to 12 genera: Glomus (10 species), Rhizophagus (2 species), Funneliformis (4 species), Claroideoglomus (1 species), Dentiscutata (2 species), Gigaspora (3 species), Scutellospora (2 species), Racocetra(1 species), Acaulospora (6 species), Entrophospora (1 species), Diversispora (1 species) and Ambispora (1) to 7 families (Glomeraceae, Claroideoglomeraceae, Gigasporaceae, Acaulosporaceae, Entrophosporaceae, Diversisporaceae, Ambisporaceae) and 3 orders (Glomerales, Diversisporales, Archaeosporales). The species Claroideoglomus etunicatum is the most common species among the studied sites; its frequency of occurrence is of the order of 12% of all isolated MA fungi.
Keywords: Diversity, endomycorrhizal fungi, rhizosphere, Acacias, Morocco