ISOLATION, SCREENING AND PHYLOGENETIC ANALYSIS OF NOVEL BACTERIA FROM ENVIRONMENTAL SAMPLES

Main Article Content

T. S. HARISH
E. ANGEL JEMIMA
P. B. RAMESH BABU

Abstract

The main focus of this paper is to isolate novel bacteria from environmental samples such as garbage waste, road side solid wastes, pond and river water samples. These samples were processed and collected in sterile containers so that it cannot be degraded. The samples were inoculated in a growth medium for the observation of wide variety of mftableicrobes in the sample. The isolated bacteria were identified for its nature of gram positive or negative and its motility. Then it is sequenced for its genome by isolating the DNA followed by phylogenetic analysis. The bacterial genome was sequenced with BigDye Terminator v3.1. Then the dendogram is done for the findings of Phylogenetic Tree. The results revealed that the isolated bacterium was Paenibacillus dendritiformis.

Keywords:
Novel bacteria, growth medium, motility, big dye terminator, dendogram

Article Details

How to Cite
HARISH, T. S., JEMIMA, E. A., & BABU, P. B. R. (2020). ISOLATION, SCREENING AND PHYLOGENETIC ANALYSIS OF NOVEL BACTERIA FROM ENVIRONMENTAL SAMPLES. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 20(23-24), 1297-1301. Retrieved from https://www.ikprress.org/index.php/PCBMB/article/view/4880
Section
Original Research Article

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