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In this study, the pathogenicity of Rhizoctonia solani recovered from diseased strawberry plants was evaluated on three strawberry varieties. Isolates H1, G1, R1 and For de R. solani were significantly highly pathogenic by inducing symptoms on the roots and crowns of inoculated plants. The severity index was maximal (100%) in Fortuna plants inoculated with H1, those of R1 and For reached 75% while those of M1 and G1 were the lowest (50%). The indexes were greater than 50% on Festival variety in response to all isolates with the exception of R2 (25%). With regard to infection ratings, the Fortuna variety was more affected by H1, R1 and For, assigned with scores of the crown rot ranging from 3 to 4. In addition, reduced development of root system of Festival variety was also observed in reponse to H1 with a disease score of 0. All the growth parameters decreased after the inocultaion experiments of the three strawberry plant varieties by the seven tested isolates of R. solani in comparison with non inoculated control plants. The infectivity reaction of strawberry plants to isolates H1, G1 and For was extensive leading to plant death after 3 weeks of inoculation. The rosette diameter of variety Fortuna inoculated by H1 was reduced to 13 cm, 14 cm by R1 and 16. 3 cm by M1 compared to 32.8 cm for control. A significant reduction in size of root part was noted in Fortuna plants inoculated with different isolates. It attained respectively 9.3 cm and 12.3 cm for G1 and H1 against 48.3 cm for control plants. Moreover, the re-isolation percentage of R. solani from roots, crown and soil for the three varieties of strawberry plants were greater than 60%, 80% and 62.5% respectively.
Bombarely AC, Merchante F, Csukasi E, Cruz-Rus JL, Caballero JL. Generation and analysis of ESTs from strawberry (Fragaria x ananassa) fruits and evaluation of their utility in genetic and molecular studies. BMC Genomics. 2010;11:503.
Anonymous. Petits fruits rouges La filière dopée par le développement récent de nouvelles productions, Revue professionnelles des filières fruits et légumes, céréalière, élevage, Agriculture du Maghreb. 2018;74:30-43.
Zhang J, Zhang L, Li GQ, Yang L, Jiang DH, Zhuang WY, Huang HC. Botrytis sinoallii: A new species of the grey mould pathogen on Allium crops in China. Mycoscience. 2010;6:421–431.
Walker AS, Gautier A, Confais J, Martinho D, Viaud M, Pecheur L, Dupont J, Fournier E. Botrytis pseudocinerea, a new cryptic species causing gray mold in French vineyards in sympatry with Botrytis cinerea. Phytopathology. 2011;12:1433–1445.
Sigee DC. Bacterial plant pathology: cell and molecular aspects. Cambridge, UK: University Press; 2005.
Golzar H, Phillips D, Mack S. Occurrence of strawberry root and crown rot in Western Australia. Australasian Plant Disease Note. 2007;2:145–147.
Juber KS, Al-Juboory HH, Al- Juboory SB. Fusarium wilt disease of strawbrry caused by Fusarium oxysporum f. sp. fragariae in Iraq and its control. Journal of Experimental Biology and Agricultural Sciences. 2014;2(4):420-427.
Martin FN. Strawberry root rot and the recovery of Pythium and Rhizoctonia spp. Alternatives and Emissions Reduction. 1999;6:1-3.
Sharon M, Freeman S, Kuninaga S, Sneh B. Genetic diversity, anastomosis groups and virulence of Rhizoctonia spp. from strawberry. European Journal of Plant Pathology. 2007;117:247-265.
Fang X, Finnegan PM, Barbetti MJ. Wide variation in virulence and genetic diversity of binucleate Rhizoctonia isolates associated with root rot of strawberry in Western Australia. Plos One. 2013;8(2): e55877.
Ceja-Torres LF, Mora-Aguilera G, Mora-Aguilera A. Agronomical management influence on the spatiotemporal progress of strawberry dry wilt in Michoacan, Mexico. African Journal of Agricultural Research. 2014;9:513-520.
Manici LM, Caputo F, Baruzzi G. Additional experiences to elucidate the microbial component of soil suppressiveness towards strawberry black root rot complex. Annals of Applied Biology. 2005;146:421–431.
Avilés M, Castillo S, Bascon J, Zea-Bonilla T, Martín-Sánchez PM, Pérez-Jiménez RM. First report of Macrophomina phaseolina causing crown and root rot of strawberry in Spain. Plant Pathology. 2008;57:382.
DOI: 10.1111/j.1365- 3059.2007.01717
Hutton DG, Gomez AO, Mattner SW. Macrophomina phaseolina and its association and Its Association with Strawberry Crown Rot in Australia. International Journal of Fruit Science. 2013;13:149-155.
Mansour AS. Pathological studies on wilt disease of Strawberry in Egypt. PhD Thesis submitted to Department of Agricultural Botany, Faculty of Agriculture, Moshtohor, Benha University, Egypt. 2005; 137.
Abdel-Sattar MA, El-Marzoky HA, Mohamed AI, Occurrence of soil borne diseases and root knot nematodes in strawberry plants grown on compacted rice straw bales compared with naturally infested soil. Journal of Plant Protection Research. 2008;48:223-235.
Mingzhu LI, Asano T, Suga H, Agevama K. A Multiplex PCR for the Detection of Phytophthora nicotianae and P. cactorum and a survey of their occurrence in strawberry production areas of Japan Plant Diseases. 2011;95:1270-1278.
Hu PBS. Effects of oilseed meals and isothiocyanates (ITCS) on Phymatotrichopsis omnivora (cotton root rot) and soil microbial communities. Thesis. Office of Graduate Studies of Texas and Nankai University. 2012;173.
Botha A, Denman S, Lamprecht SC, Mazzola M, Crous PW. Characterization and pathogenicity of Rhizoctonia isolates associated with black root rot of strawberries in the Western Cape Province, South Africa. Australasian Plant Pathol. 2003;32:195-201.
Fu-chun Z, Yun H, Yan-qin Z, Shu-qing MA. Biological characteristics of strawberry Fusarium Wilt. Journal of Sichuan Agricultural University.
(Access on June 21, 2014)
Nagarajan G, Kang SW, Nam MH, Song JY, Yoo SJ, Kim HG. Characterization of Fusarium oxysporum f. sp. fragariae based on vegetative compatibility group, random amplified polymorphic DNA and pathogenicity. Journal of Plant Pathology 2006;22:222–229.
Suga H, Hirayama Y, Morishima M, Suzuki T, Kageyama K, Hyakumachi M. Development of PCR primers to identify Fusarium oxysporum f. sp. fragariae Plant Disease. 2013;97:619-625.
Koike ST, Kirkpatrick SC, Gordon TR. Fusarium wilt of strawberry caused by Fusarium and Macrophomina in California. Plant Disease. 2009;93:1077.
Manici LM, Bonora P. Molecular genetic variability of Italian binucleate Rhizoctonia spp. isolates from strawberry. European Journal of Plant Pathology. 2007;118:31–42.
Williamson M, Fernández-Ortuño D, Schnabel G. First Report of Fusarium wilt of strawberry caused by Fusarium oxysporum in South Carolina. Plant Disease. 2012;96:911.
Mouden N, Al Batnan A, Benkirane R, Ouazzani Touhami A, Douira A. Diversity and distribution of fungi from strawberry plants grown in Gharb-Loukkos (Morocco). International Journal of Recent Scientific Research. 2016a;7(10):13630-13641.
Al Batnan A, Mouden N, Salim S, Chliyeh M, Ouazzani Touhami A, Douira A. Fungal communities in roots, soil inhabiting nematodes and physico-chemical parameters of soils in three farms of commercial strawberry production in Moulay bousselham (Morocco). Annual Research & Review in Biology. 2018;28(5):1-16.
Mouden N, Benkirane R, Ouazzani Touhami A, Douira A. Fungal species associated with collapsed strawberry plants cultivated in strawberries plantations in Morocco. International Journal of Current Research. 2016b;8(4):29108-29117.
Aoyagi K, Ross PD, Davis JW, Wasnich RD, Hayashi T, Takemoto T. Falls among community-dwelling elderly in Japan. J. Bone Miner. Res. 1998;13:1468-1474.
Martin SB. Identification, isolation frequency and pathogenicity of anastomosis groups of binucleate Rhizoctonia spp. from strawberry roots. Phytopathology. 1988;78: 379-384.
Abad ZG, Louws FJ, Fernandez GE. Rhizoctonia and Pythium species associated with black root rot of strawberries in North Carolina. Phytopathology. 1999;89(6):S1 (Abstr.).
Juber KS, Al-Juboory HH, Al-Juboory SB. Identification and control of strawberry root and stalk rot in Iraq. International Journal of Environmental & Agriculture Research. 2016;2(2):54-63.
Mouden N, Benkirane R, Ouazzani TA, Douira A. Mycoflore de quelques variétés du fraisier (Fragaria ananassa L.), cultivées dans la région du Gharb et le Loukkos (Maroc). Journal of Applied Biosciences. 2013;61:4490–4514.
Botha A, Denman S, Crous PW, Lamprecht SC. Strawberry diseases: Methy bromide out-black root rot in! Deciduous Fruit Grower. 2001;51:25-27.
Christlyn AP, Hancock JF. Field evaluation of strawberry genotypes for tolerance to black root rot on fumigated and non fumigated soil. J. Amer. Soc. Hort. Sci. 2005;130(5):688-693.
Matsumoto M, Yoshida T. Characterization of isolates of binucleate Rhizoctonia spp. associated with strawberry black root rot complex using fatty acid methyl ester (FAME) profiles. Journal of General Plant Pathology. 2006;72(5):318-322.
Carling DE, Sumner DR. Rhizoctonia. In: Methods for research on soilborne phyopathogenic fungi. Singleton LL, Mihail JD, Rush CM. eds. American Phytopathological Society, St. Paul, MN. 2001;157-165.
Xi K, Stephens JHG, Hwang SF. Dynamics of pea seed infection by Pythium ultimum and Rhizoctonia solani: effects of inoculum density and temperature on seed rot and preemergence damping-off. Can. J. Plant Pathol. 1995;17:19-24.
Uz-Zaman AM, Bhuiyan MR, Khan MA, Alam Bhuiyan MK, Latif MA. Integrated options for the management of black root rot of strawberry caused by Rhizoctonia solani Kuhn. Plant biology and pathology/Biologie et Pathologie Végétales. 2015;338(2):112-20.
Ahmed MFA, El-Fiki IAI. Effect of biological control of root rot diseases of strawberry using Trichoderma spp. Middle East Journal of Applied Sciences. 2017;03:482-492.
Erper I, Ozkoc I, Karaca GH. Identification and pathogenicity of Rhizoctonia species isolated from bean and soybean plant in Samsun, Turkey. Archives of Phytopathology and Plant Protection. 2011;44(1):78–84.
Yuen GY, Craig ML, Giesler LJ. Biological control of Rhizoctonia solani on tall fescue using fungal antagonists. Plant Disease. 1994;78:118–123.
Eken C, Demirci E. Anastomosis groups and pathogenicity of Rhizoctonia solani and binucleate Rhizoctonia isolates from bean in Erzurum, Turkey. Journal of Plant Pathology. 2004;86(1):49-52.
Gurkanli CT, Ozkoc I. First Report of B.N. Rhizoctonia from tobacco (Nicotiana tabacum L.) in Samsun, Turkey. Pakistan Journal of Botany. 2011;43(1):51-57.
Hua GKH, Bertier L, Soltaninejad S, Hofte M. Cropping systems and cultural practices determine the Rhizoctonia anastomosis groups associated with Brassica spp. in Vietnam. Plos One. 2014;9(11):e111750.
Unal F, Dolar FS, Yildirim F, Demirci E. Isolation and identification of binucleate Rhizoctonia spp. from wheat field soils in the Central Anatolia Region, Turkey. Turkish Journal of Agricultural and Natural Sciences. 2014;SI2:1933-1938.
Muzhinji N, Truter M, Woodhall JW, Van der Waals JE. Anastomosis groups and pathogenicity of Rhizoctonia solani and binucleate Rhizoctonia from potato in South Africa. Plant Disease. 2015;99:1790-1802.
Sharma PD, Paulitz TC, Porter LD, Du Toit LJ. Characterization and pathogenicity Rhizoctonia and Rhizoctonia- like spp. from pea crops in Columbia Basin of Oregon and Washington. Plant Disease. 2015;99:604-613.
Vojvodić Mira, Tanović B, Mihajlović M, Mitrović P, Vico I, Bulajić A. Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia. Pestic. Phytomed. (Belgrade). 2018;33(2): 97-107.
Errifi A, Ouazzani TA, Mouden N, Chliyeh M, Al Batnan A, Selmaoui K, Benkirane R, Douira A. Pathogénie comparée de Rhizoctonia solani sur différentes espèces végétales cultivées au Maroc. Plant archives. 2019;19.
Kim WG, Cho WD, Lee YH. Anastomosis groups and pathogenicity of Rhizoctonia solani isolates from strawberry (Fragaria ananassa Duch.). Korean J. Plant Pathol. 1992;8(2):91-95.
Tanaka MADS, Ito MF, Passos FA. Pathogenicity of Rhizoctonia solani to strawberry. Bragantia. 1995;54(2):319-324.
Fang XL, Phillips D, Sivasithamparam HLIK, Barbetti MJ. Severity of crown and root diseases of strawberry and associated fungal and oomycete pathogens in Western Australia. Australasian Plant Pathology. 2011;40:109-119.
Paulitz TC, Smith JD, Kidwell KK. Virulence of Rhizoctonia oryzae on wheat and barley cultivars from the Pacific northwest. Plant Disease. 2003;87:51- 55.
Schroeder KL, Paulitz TC. Effect of inoculum density and soil tillage on the development and severity of Rhizoctonia root rot. Phytopathology. 2008;98:304- 314.
Silva DMP, Tylka GL, Munkvold GP. Seed treatment effects on maize seedlings coinfected with Rhizoctonia solani and Pratylenchus penetrans. Plant Disease. 2017;101:957-963.
Kucharska K, Katulski B, Goriewa K, Duba A, Wachowska U. Pathogenicity and fungicide sensitivity of Rhizoctonia solani and R. cerealis isolates. Gesunde Pflanzen. 2018;70:13.