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Zingiber zerumbet (L.) Sm. is well known as pinecone lily, bitter ginger and is wild ginger belonging to the Zingiberaceae family. The present study is aimed to develop an efficient protocol for micropropagation through the culture of rhizome bud explants. Murashige and Skoog (MS) basal medium supplemented with different concentrations and combinations of 6-benzyl amino purine (BAP), kinetin (Kn) and α- naphthalene acetic acid (NAA) were employed towards the induction and effective multiplication of shoots. The highest percentage of shoot induction was observed on the medium supplemented with 8.88 µM/L of BAP and 1.10 µM/L of NAA. The maximum number of micro shoots (5.10 ± 0.31) was achieved on the medium fortified with 8.88 µM/L of BAP and 1.10 µM/L of NAA. The initiated shoots were subcultured on fresh medium enriched with the combination of BAP+NAA, Kn+NAA and BAP+Kn+NAA. Among the various combinations studied, the medium fortified with 8.88 µM/L of BAP, 9.30 µM/L of Kn and 1.10 µM/L of NAA was found to be the most suitable one towards the maximum percentage of shoot multiplication with the highest number of shoots (13.40 ± 0.47) and mean shoot length of 9.01 ± 0.17 cm. The multiple shoots produced roots spontaneously in the multiplication stage itself. The healthy shoot lets were hardened and transfer to the field with 100% of survival. The developed protocol is the most suitable one for the propagation of shoots and roots simultaneously. Hence this could be useful for the large scale production of plantlets of this important medicinal plant.

Zingiber zerumbet, micropropagation, rhizome buds, wild ginger.

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Original Research Article


Rout Om Prakash, Acharya Rabinarayan, Mishra Sagar Kumar. Zingiber zerumbet (L.) Sm., a reservoir plant for therapeutic uses; A Review. International Journal of Pharma World Research. 2011;2(2):1-22.

Anonymous. The wealth of India. Raw materials. X-Z. India, CSIR. 1976;11:89-90.

Moshrafuddin Ahmed. Medicinal plants. M.J.P. Publishers, Chennai, India. 2010;5-6.

Joy PP, Thomas J, Mathew S, Skaria BP. Zingiberaceous medicinal and aromatic plants. Aromatic and Medicinal plants Research Station, Odakkali, Asamannoor P.O., Kerala, India; 1998.

Thakur RS, Puri HS, Husain A. Major medicinal plants of India, CIMAP, Lucknow, India. 1989;50-52.

Faizah S, Somchit MN, Shukriyah MH. Zingiber zerumbet (Lempoyang): A potential anti-inflammatory agent. Proceedings of the Regional Symposium on Environ. Nat. Res. Kula Lumpur, Malaysia. 2002;1:516-520.

Ong HC, Norzalina J. Malay herbal medicine in Germancheh Negeri Sembalan, Malaysia, Fitoterapia. 1999;70(1):10-14.

Zakaria ZA, Mohamad AS, Ahmad MS, Mokhtar AF, Israf DA, Lajis NH, Sulaiman MR. Preliminary analysis of the anti inflammatory activity of essential oil of Zingiber zerumbet. Biological Research for Nursing. 2011;13:425-432.

Sharifah Sakinah S, Tri Handayani S, Azimahtol Hawariah L. Zerumbone induced apoptosis in liver cancer cells via modulation of Bax/Bcl-2 ratio. Cancer Cell. 2007;7:4.

Murakami A, Takahashi D, Kinoshita T, Koshimizu K, Kim HW, Yoshihiro A, Nakamura Y, Jiwajinda S, Terao J, Ohigashi H. Zerumbone, a southeast Asian ginger sesquiterpene, markedly suppresses free radical generation, proinflammatory protein production and cancer cell proliferation accompanied by apoptosis: The alpha, beta-unsaturated carbonyl group is a prerequisite. Carcinogenesis. 2002; 23(5):795-802.

Abbott IA. La’au Hawai’i: Traditional Hawaiian uses of plants, Bishop Museum Press, Honolulu, Hawaii, USA; 1992.

Chun MN. Native Hawaiian medicine, First People’s Productions, Honolulu, Hawaii, USA; 1994.

Pandey BP. Economic botany, published by Chand and company, New Delhi. 2011; 293-294.

Das PC. Economic botany, AITBS Publishers, India. 2009;174.

Dikshit VK. Export of medicinal plants from India need for resource management. In: Biodiversity – North – East - India perspectives: Peoples participation in Biodiversity conservation. (Eds.). Kharbuli B, Syem D, Kayang H. NEBRC, North-Eastern Hill University, Shillong. 1999;85-88.

Dhar U, Manijkhol S, Joshi M, Ghatt A, Bisht AK. Current status and future strategy for development of medicinal plants sector in Uttaranchal, India. Current. Science. 2002;83:956-964.

Schmincke KH. Medicinal plants for forest conservation and healthcare. Non-Wood Forest Products 11, Food and Agriculture Organization of the United Nations; 2003.

Bhattacharjee SK. Hand book of medicinal plants. Fifth revised and enlarged edition, Shashi Jain for Pointer Publishers, Jaipur, India. 2008;4-6.

Rahman H, Karuppaiyan R, Kishore K, Denzongpa R. Traditional practices of ginger cultivation in northeast India. Indian Journal of Traditional Knowledge. 2009; 8(1):23-28.

Nayak S, Naik PK. Factors effecting in vitro microrhizome formation and growth in Curcuma longa L. and improved field performance of micropropagated plants. Science Asia. 2006;32:31-37.

Hosoki T, Sagawa Y. Clonal propagation of ginger (Z. officinale Rosc.) through tissue culture. Horticultural Sciences. 1977;12: 451-452.

Hamirah MN, Sani HB, Boyce PC, Sim SL. Micropropagation of red ginger (Zingiber montanum Koenig), a medicinal plant. AsPac J. Mol. Biol, Biotechnol. 2010; 18(1):127-130.

Poonasapaya P, Kraisintu K, Paleitech D, Puteusky E. Micropropagation of Zingiber cassumunar Roxb. Acta Horticulturae. 1993;344:557-564.

Sajc L, Grubisic D, Vunjak-Novakovic G. Bioreactors for plant engineering: An outlook for further research. Biochem. Engng J. 2000;4:89-99.

Tiwari Bhrigu Narayan, Khatri Pankaj, Ali Julfikar, Soni ML, Pael Rakesh. Tissue culture of endangered Bael tree (Aegle marmelos); A review. J. Adv. Sci. Res. 2010;1(2):34-40.

Fakhrai HK, Fakhrai F. Hormonal control of growth and development. In: Pollard, J W & Walker, J M (eds) Methods in Molecular Biology, Plant cell and Tissue culture. Humana Press, New Jersey. 1990; 6:49-56.

Faridah QZ, Abdelmageed AHA, Julia AA, Nor Hafizah R. Efficient in vitro regeneration of Zengiber zerumbet Smith. (a valuable medicinal plant) Plantlets from rhizome bud explants. African Journal of Biotechnology. 2011;10(46):9303-9308.

Rakkimuthu R, Jinu Jacob, Aravinthan KM. In vitro micropropagation of Alpinia zerumbet variegate, an important medicinal plant, through rhizome bud explants. Research in Biotechnology. 2011;2(1):07-10.

Melati Yulia Kusumastuti, Arvind Bhatt, Gunawan IndraYanto, Chan Lai Keng. Effect of sucrose, benzylamino purine and culture condition on in vitro propagation of Curcuma xanthorrhiza Roxb. and Zingiber aromaticum Val. Pak. J. Bot. 2014;46(1): 279-288.

Orawan Theanphong, Thanapat Songsak, Chalermpol Kirdmanee. Effect of plant growth regulators on micropropagation of Curcuma aeruginosa Roxb. Thai Journal of Botany. 2010;2:135-142.

Shahinozzaman M, Faruq MO, Mustafa Abul Kalam Azad, Muhammad Nurul Amin. Studies on in vitro propagation of an important medicinal plant- Curcuma zedoaria roscoe using rhizome explants. Persian Gulf Crop Protection. 2013;2(4):1-6.

Philip Robinson J, John Britto S, Balakrishnsn V. Micropropagation of Costus speciosus (Koem.ex.retz) Sm., An antidiabetic plant by using explants of Pseudostems. Botany Research Inter-national. 2009;2(3):182-185.

Gamborg OL, Phillips GC. Plant cell tissue and organ culture. Fundamental methods, Narosa Publishing House, New Delhi; 2004.

Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962; 15:473-497.

Habibi N, Suthar RK, Purohit SD. Role of PGRs and inhibitors in induction and control of somatic embryogenesis in Themeda quadrivalvis. Indian J. Exp. Biol. 2009;47:198.

Ayenew B, Tefera W, Kassahun B. In vitro propagation of Ethiopian ginger (Zingiber officinale Rosc.) cultivars: Evaluation of explant types and hormone combinations. African Journal of Biotechnology. 2012; 11(16):3911-3918.

Shagufta N, Saiqa I, Sumera Aamir A. In vitro clonal multiplication and acclimatization of different varieties of Turmeric (Curcuma longa L.). Pak. J. Bot. 2009;4(6):2807-2816.

Raju B, Anita D, Kalita MC. In vitro clonal propagation of Curcuma caesia Roxb and Curcuma zedoaria Rosc. From rhizome bud explants. Journal of Plant Biochem and Biotechnology. 2005;14:61-63.

Chirngini P, Sinha SK, Sharma GJ. In vitro propagation and micro rhizome induction in Kaempferia galangal L. and K. rotunda L. Indian Journal of Biotechnology. 2005;4: 404-408.

Sangamitra N. In vitro multiplication and micro rhizome induction in Curcuma aromatic Sallsb. Plant Growth Regulation. 2000;32:41-42.

Parrot WA, Bailey MA, Durham RE, Mathews HV. Biotechnology and crop improvement in Asia. In: Moss JP (ed) Biotechnology and crop improvement in Asia. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India. 1992;115-148.

Mneny EE, Mantell SH. Clonal propagation of cashew (Anacardium occidentale L.) by tissue culture. J. Hor.t Sci. Biotech. 2002; 77:649-657.

Yusuf NA, Suffian Annur MM, Khalid N. Rapid micropropagation of Boesenbergia rotunda (L.) Mansf. Kulturpfl. (a valuable medicinal plant) from shoot bud explants. African Journal of Biotechnology. 2011; 10(7):1194-1199.

Kambaska KB, Santilata S. Effect of plant growth regulator on micropropagation of ginger (Gingiber officinale Rocs.) cv- Suprava and Suruchi. Journal of Agricultural technology. 2009;5(2):271-280.

Hashimah HM, Ibrahim H, Rahim Z. Preliminary studies on some nutritional composition of the edible gingers. In Advance in Aiochemistry and Biotechno-logy in Asia and Oceania. Procedings of the 7th Federation of Asia and Oceania Bio-chemists Symposium (FAOB). El-2; 1998.

Hoque MI, Perveen S, Sarker HR. In vitro propagation of ginger. (Zingiber officinales Rocs.) Plant Tissue Cult. 1977;9:45-51.

Noguchi Y, Yamakawa O. Rapid clonal propagation of ginger (Zingiber officinales Rosc.). Japan J. Breed. 1998;38:437-442.

Loc NH, Duc DT, Kwon HT, Yang MS. Micropropagation of Zedoary (Curcuma zedoaria Roscoe.) a valuable medicinal plant. Plant Cell Tiss. Org. Culture. 2005; 81:119-122.

Stanley C, Keng CL. Micropropagation of Cucurma zedoaria Rosc. and Zingiber zerumbet Smith. Biotechnol. 2007;6(4): 555-560.

Nayak S. In vitro multiplication and microrhizome induction in Curcuma amomatica Salisb. Plant Growth Regul. 2000;32:41-47.

Abdelmageed AHA, Faridah QZ, Norhana FMA, Julia AA, Midhzar Abdul Kadir. Micropropagation of Etlingera elatior (Zingiberaceae) by using axillary bud explants. Journal of Medicinal Plants Research. 2011;5(18):4465-4469.

Selvakumar C, Balakrishnan A, Subhadra Lakshmi B. Rapid in vitro micro-propagation of Alpinia officinarum hence, an important medicinal plant, through rhizome bud explants. Asian Journal of Plant Sciences. 2007;6(8):1251-1255.

Sharma TR, Singh BM. High-frequency in vitro multiplication of disease-free Zingiber officinale Rosc. Plant Cell Rep. 1997;17: 68-72.

Sriskandarajah S, Skirvin RM, Abu-Gaoud H, Korban SS. Factors involved in shoot elongation and growth of adventitious and axillary shoots of three Scion apple cultivars in vitro. J. Hortic. Sci. 1990;65: 113-121.

Anderson WC. Micropropagation of filberts, Corylus avellono. Camb. Proc. Int. Plant Prop Soc. 1984;33:132-137.

Sen J, Sharma AK. Micropropagation of Withania somnifera from germinated seeds and shoot tips. Plant Cell Tiss. Org. Culture. 1991;26:71-73.

Coenen C, Lomax TC. Auxin-cytokinin interaction in higher plants: Old problem and new tools. Trends in Plant Science. 1997;2(9):351-356.

Torrey JG. Physiological basis of organization and development in the root. In: Ruhland W (ed) Encyclopedia of plant physiology. Springer, Berlin; 1965;15(1).

Torrey JG. Root hormones and plant growth. Ann Rev Plant Physiol. 1976;27: 435-459.

Scott TK. Auxins and roots. Ann Rev Plant Physiol. 1972;23:235-258.

Goncalves JC, Diogo G, Amancio S. In vitro propagation of chestnut (Castanea sativa x C. crenata): Effect of rooting treatments on plant survival, peroxidase activity and anatomical changes during adventitious root formation. Scientia Horticulturae. 1998;72:265-275.

Dogra SP, Koria BN, Sharma PP. In vitro clonal propagation of ginger (Zingiber officinale Rosc.) Horticultural Journal. 1994;7:45-50.

Meenakshi N, Sulikeri GS, Krishnamoorthy V, Ramakrishnan VH. Standardization of chemical environment for multiple shoot induction of turmeric (Curcuma longa L.) for in vitro clonal propagation. Crop Research. 2001;22:449-453.

Balachandran SM, Bhat SR, Chandel KPS. In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Z. officinale Rosc.). Plant Cell Rep. 1990;8:521- 529.

Chan LK, Thong WH. In vitro propagation of Zingiberaceae species with medicinal properties. J. Plant Biotechnol. 2004;6:181-188.

Bharalee R, Das A, Kalita MC. In vitro clonal propagation of Curcuma ceasia Roxb. and Curcuma zedoaria Rosc. From rhizome bud explants. J. Plant Biochem. Biotechnol. 2005;14:61-63.

Nadgauda RS, Mascarenhas AF, Hendre RR, Jagannathan V. Rapid multiplication of turmeric (Curcuma longa Linn.) plant by tissue culture. Indian J. Exp. Biol. 1978;16: 120-122.

Prathanaturarug S, Soonthornchareonnon N, Chuakul W, Phaidee Y, Saralamp P. Rapid micropropagation of Curcuma longa using bud explants pre-cultured in Thidiazuron-supplemented liquid medium. Plant Cell Tissue and Organ Culture. 2005;80:347-351.

Ferdous MM, Shahinozzaman M, Faruk MO, Paul SP, Azad MAK, Amin MN. In vitro propagation of a medicinal plant- mango ginger (Curcuma amada Roxb.). International Journal of Bio Science. 2012; 2(11):166-172.

Inden H, Asahira T, Hirano A. Micro-propagation of ginger. Acta Hortic, 1988; 230:177-184.