CHARACTERIZATION AND CROSSING TWO CULTIVARS OF OKRA (Abelmoschus esculentus L.) FOR CROP IMPROVEMENT IN EGYPT
ZAINAB G. AHMED
Department of Botany, Faculty of Sciences, Aswan University, Aswan 81528, Egypt.
MAGDI A. EL-SAYED *
Department of Botany, Faculty of Sciences, Aswan University, Aswan 81528, Egypt and Unit of Environmental Studies and Development, Aswan University, Aswan 81528, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Genetic diversity and relatedness in crop germplasm is important to design strategies to improve traits and evaluate new varieties in okra by using the appropriate breeding technique. Okra (Abelmoschus esculentus L.) is an important multi-purpose vegetable crop cultivated and consumed across all tropical and temperate regions of the world. Two cultivars, Egyptian and Japanese, having a broad genetic background and phenotypic diversity were chosen as parents and were crossbred in both directions to assess intra-specific hybridization between these two cultivars. A significant variation between parents and corresponding hybrids was found in morphological characters including qualitative and quantitative characters such as main stem height, internode length, leaf area, petiole color, fruit color and fruit pubescence. The F1 hybrid plants were intermediate showing the dominant characters. They were tall with average height (80.3 cm), having red color petiole and fruit. The anthocyanin contents were higher in Egyptian mothers (103 mg/g FW) followed by Egyptian cultivar × Japanese cultivar (62.84 mg/g DW) and Japanese cultivar × Egyptian cultivar (46.07 mg/g DW). Petal blotch is on both sides similar to Japanese mother, while in Egyptian mothers petal blotch positioned inside only. Physiological attributes and metabolite contents were also assessed including total chlorophyll content, ascorbic acid, phenolics, flavonoids, carbohydrates, protein and saponins. The results indicated that the two intra specific hybrids consist of genetic material from both Egyptian and Japanese cultivars. The F1 hybrid characteristics were better and possessed desirable characters confirming that crossbreeding could be a useful tool for okra crop improvement.
Keywords: Abelmoschus esculentus, crossbreeding, evaluation, genetic diversity, metabolites, morphological attributes.
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Dhankhar BS, Mishra JP. Objectives of Okra Breeding. J. New Seeds. 2005;6:2-3, 195-209.Martin FW. A second edible okra species, and its hybrids with common okra. Ann. Bot. 1982;50:277-283.
Lamont Jr. WJ. Okra - A versatile vegetable crop. Hort. Technol. 1999;9(2):179-184.
Gulsen O, Karagul S, Abak K. Diversity and relationships among Turkish germplasm by SRAP and Phenotypic marker poly-morphism. Biologia. 2007;62(1):41-45.
Kiran PTS, Ravisankar C. Genetic variability and multivariate analysis in okra [Abelmoschus esculentus (L.) Moench]. Trop. Agri. Res. 2004;16:99-113.
Yonas M, Garedew W, Debela A. Multivariate Analysis among Okra (Abelmoschus esculentus (L.) Moench) Collection in South Western Ethiopia. J. Plant. Sci. 2014;9(2):43-50.
Beck DL, Vasal SK, Crossa J. Heterosis and combining ability of CIMMYT’s tropical early and intermediate maturity maize germplasm. Maydica. 1990;35:279–285.
Vasal SK, Cordova H, Beck DL, Edmeades GO. Choices among breeding procedures and strategies for developing stress tolerant maize germplasm. Maydica. 1997;35:279–285.
Adeniji OT. Inheritance studies in West African okra (A. caillei). M. Agric. Thesis. University of Agriculture. Abeokuta, Nigeria, 2003;98.
Seck A. Breeding procedures and results on indigenous vegetalbles: Examples of African eggplant solanum aethiopicum and okra Abelmoschus spp. Acta Hortic. 2000; 522:195-208.
IPGRI. Okra Descriptor list.International Crop Network Series 5. International Board for Plant Genetic Resources (IBPGR), Rome, Italy; 1991.
Ni Z, Kim ED, Chen ZJ. Chlorophyll and starch assay. Protocol exchange; 2009.
DOI: 10.1038/nprot.2009.12
Lichtenthaler H, Wellburn A. Determinations of total carotenoids and chlorophyllsb of leaf extracts in different solvents. Biochem. Soc. Trans. 1983;11: 591–592.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J. Biol. Chem. 1951; 193:265–275.
Fales FW. The assimilation and degradation of carbohydrates by yeast cells. J. Biol. Chem. 1951;193:113.
Trevelyan WE, Forrest RS, Harrison JS. Determination of yeast carbohydrates with the anthrone reagent. Nature 1952;170:626–627.
Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug. Anal. 2002;10:178–182.
Vu HQ, El-Sayed MA, Ito S, Yamauchi N, Shigyo M. Discovery of a new source of resistance to Fusarium oxysporum, cause of Fusarium wilt in Allium fistulosum, located on chromosome 2 of Allium cepa Aggregatum group. Genome. 2012;55(10): 797-807.
Ebrahimzadeh H, Niknam V. A revised spectrophotometric method for determination of Triterpenoid saponins. Indian Drugs. 1998;35:379–382.
Bates LS, Waldran RO, Terare ID. Rapid determination of free proline for water stress studies. Plant soil. 1973;39:205- 208.
Omaye ST, Turnbull, JD, Sauberilick HE. Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. Methods enzymol. 1979;62:3-11.
Ough CS, Amerine MA. Methods for analysis of must and wines. 2nd ed. New York: John Wiley & Sons; 1988.
Oyetunde OA, Ariyo OJ. Genetics of seed yield and related traits in biparental crosses of okra, Abelmoschus esculentus (L.) Moench. Niger. J. Gen. 2014;28:8e14.
Ilodibia1 CV, Achebe UA, Aziagba BO, Okeke NF, Udeorah SN. Effects of crossbreeding on fruits characteristics of two species of tomatoes (Solanum esculentum L. and Solanum pimpinellifolium L.). Arch. Agr. Environ. Sci. 2017;2(4): 305-307.
Mclendon CA. Mendelian inheritance in cotton hybrids. Ga. Agr. Exp. Sta. Bull. 1912;99:141-228.
Miller JC, Wilson WF. A preliminary report on okra breeding in Louisiana. Proc. Amer. Soc. Hort. Sci. 1938;35:551-553.
Zečević V, Knežević D, Mićanović D, Pavlović M, Urošević D. The inheritance of plant height in winter wheat (Triticum aestivum L.). Genetika. 2005;37(2):173-179.
Doku AH. Characterization of 18 African rice (Oryza glaberrima) in Ghana. An (MPhil.) Thesis, Department of Nuclear Agriculture and Radiation Processing, University of Ghana. 2011;87-102.
Dixit R, Shukla N, Verma S. Evaluation of studies of different varieties/ hybrids of okra [Abelmoschus esculentus (L.) Moench] under Chhattisgarh region. Intl. J. Chem. Stud. 2018;6(6):2442-2445.