STUDY OF THE EFFECT OF DIFFERENT PHOSPHO-COMPOST DOSES ON THE GROWTH AND DEVELOPMENT OF BEAN PLANTS
S. EL GABARDI
Laboratoire de Botanique Biotechnologie et de Protection des Plantes, Faculté des Sciences, Université Ibn Tofail, Kenitra, Morocco.
M. CHLIYEH
Laboratoire de Botanique Biotechnologie et de Protection des Plantes, Faculté des Sciences, Université Ibn Tofail, Kenitra, Morocco.
N. MOUDEN
Laboratoire de Botanique Biotechnologie et de Protection des Plantes, Faculté des Sciences, Université Ibn Tofail, Kenitra, Morocco.
K. SELMAOUI
Laboratoire de Botanique Biotechnologie et de Protection des Plantes, Faculté des Sciences, Université Ibn Tofail, Kenitra, Morocco.
A. OUAZZANI TOUHAMI
Laboratoire de Botanique Biotechnologie et de Protection des Plantes, Faculté des Sciences, Université Ibn Tofail, Kenitra, Morocco.
C. EL MODAFAR
Laboratoire de Biotechnologie et Bio-ingénierie Moléculaire, Faculté des Sciences et Techniques, Université Cadi Ayyad, Marrakech, Morocco.
A. FILALI MALTOUF
Laboratoire de Microbiologie et Biologie Moléculaire, Faculté des Sciences, Université Mohammed, Rabat, Morocco.
S. EL ABED
Laboratoire de Biotechnologie Microbienne, Faculté des Sciences et Techniques, Université Sidi Mohamed Ben, Abdellah, Fès, Marocco.
S. IBNSOUDA KORAICHI
Laboratoire de Biotechnologie Microbienne, Faculté des Sciences et Techniques, Université Sidi Mohamed Ben, Abdellah, Fès, Marocco.
S. AMIR
Laboratoire Polyvalent en Recherche et Développement, Université Sultan Moulay Slimane, Morocco.
A. MOUKHLI
Unité de Recherche d’Amélioration Génétique des Plantes, Institut National de la Recherche Agronomique, Marrakech, Morocco.
R. BENKIRANE
Laboratoire de Botanique Biotechnologie et de Protection des Plantes, Faculté des Sciences, Université Ibn Tofail, Kenitra, Morocco.
A. DOUIRA *
Laboratoire de Botanique Biotechnologie et de Protection des Plantes, Faculté des Sciences, Université Ibn Tofail, Kenitra, Morocco.
*Author to whom correspondence should be addressed.
Abstract
The objective of this study is to demonstrate the effect of different doses of phospho-compost (2.5%; 5%; 7.5%; 10%) on the growth and development of the bean plants and to see the ability of endomycorrhizal fungi contained in sludge, an essential component of this phospho-compost, to mycorrhize these seedlings. The obtained results showed that the 2.5% and 5% doses are the most favorable for plant growth and development. At the 2.5% phospho-compost dose, compared with the control, stem length, number of leaves, flowers, and pods are highest, 46 and 22.9 cm, 17 and 11 leaves per plant, 9 and 2 flowers per plant, and 6 and 2 pods per plant. At the 5% dose, these parameters are respectively 40 cm, 15 leaves / plant, 6 flowers / plant, 5 and pods / plant. The values of the different parameters recorded decrease in plants growing on phospho-compost doses of 7.5 and 10%. The bean plants growing on different doses of phospho-compost have contracted an association with arbuscular mycorrhizae; different structures of these fungi have been noted: intra and extraradicular hyphae, vesicles, arbuscules and spores. The intraradicular spore content was estimated for the first time by adopting the used formulas to estimate vesicle and arbuscular contents. The highest spore levels were found in the roots of plants growing on substrates containing phospho-compost doses of 2.5 and 5%, respectively 73.3 and 60.5%. Similarly, morphotypes of endomycorrhizal fungi are isolated from the rhizosphere of bean plants growing on different doses of phospho-compost. These morphotypes are representatives of three genera: Glomus, Acaulospora, and Scutellospora.
Keywords: Phospho-compost, dose, bean, Arbuscular Mycorrhizal Fungi (AMF), agronomic parameters, intraradicular spores, rhizosphere.